BPGM Antibody

2,3-Bisphosphoglycerate Mutase (BPGM) is a multifunctional enzyme that plays a crucial role in the regulation of hemoglobin oxygen affinity by controlling the levels of its allosteric effector, 2,3-bisphosphoglycerate (2,3-BPG). This enzyme is encoded by the BPGM gene and is involved in both the synthesis and degradation of 2,3-BPG .

BPGM is a member of the phosphoglycerate mutase family and exists as a homodimer. It catalyzes the conversion of 3-phospho-D-glyceroyl phosphate to 2,3-bisphospho-D-glycerate (2,3-BPG), a reaction essential for controlling the concentration of 2,3-BPG within the cell . The enzyme exhibits mutase activity (EC 5.4.2.11) and has phosphoglycerate phosphomutase activity .

2,3-BPG is a small molecule found at high concentrations in red blood cells, where it binds to hemoglobin and decreases its oxygen affinity. This binding is crucial for the efficient release of oxygen to tissues. BPGM’s role in regulating 2,3-BPG levels is therefore vital for maintaining proper oxygen delivery throughout the body .

The BPGM gene is located on chromosome 7 and has multiple alternatively spliced variants encoding the same protein . Mutations in this gene can lead to hemolytic anemia, a condition characterized by the destruction of red blood cells . Additionally, BPGM is associated with diseases such as Erythrocytosis, Familial, 8, and Autosomal Recessive Secondary Polycythemia .

Mouse anti-human BPGM antibodies are monoclonal antibodies used in various research applications, including Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) . These antibodies are specific for an epitope within the internal region of the human BPGM protein and are used to detect BPGM expression in different tissues and cell types .

Research on BPGM has significant implications for understanding and treating conditions related to oxygen delivery and red blood cell function. The enzyme’s role in hemoglobin regulation makes it a potential target for therapeutic interventions in diseases such as anemia and polycythemia .