Borrelia Spielmanii OspC
Greater than 95.0% as determined by SDS-PAGE.
Description
Recombinant Borrelia Spielmanii Outer Surface Protein C produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 24kDa.
Borrelia Spielmanii OspC is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
This product consists of recombinant Borrelia Spielmanii Outer Surface Protein C (OspC), produced in E. coli. This non-glycosylated polypeptide has a molecular weight of 24kDa and features a -6x His tag at the N-terminus. It undergoes purification using proprietary chromatographic methods.
This product is supplied in a buffer solution containing 20mM HEPES (pH 8), 200mM NaCl, and 20% glycerol.
The purity level exceeds 95%, as determined by SDS-PAGE analysis.
2. Applicable for immunodot assays using Lyme disease positive and negative plasma samples. Suitable for use in lymphocyte transformation tests (LTT).
Suitable for use in Western blot analysis with patient samples.
Product Science Overview
Borrelia spielmanii is a species of spirochete bacteria belonging to the Borrelia burgdorferi sensu lato complex, which is known to cause Lyme disease. One of the key proteins expressed by Borrelia species during infection is the Outer Surface Protein C (OspC). This protein plays a crucial role in the early stages of infection and is a target for diagnostic and therapeutic research.
Borrelia spielmanii is one of the several Borrelia species that can cause Lyme disease in humans. It is primarily transmitted through the bite of infected Ixodes ticks. The bacteria are known for their ability to evade the host immune system and establish long-term infections.
OspC is a surface-exposed lipoprotein that is highly expressed by Borrelia species during the early phase of infection. It is involved in the initial stages of host infection and is essential for the bacteria’s survival in the mammalian host. OspC facilitates the dissemination of Borrelia from the tick bite site to various tissues in the host by binding to host proteins such as fibrinogen .
Recombinant OspC refers to the OspC protein that has been produced using recombinant DNA technology. This involves cloning the ospC gene from Borrelia spielmanii into an expression vector, which is then introduced into a host organism, typically E. coli, to produce the protein. The recombinant OspC protein is often tagged with a His-tag to facilitate purification .
- Diagnostic Use: Recombinant OspC is used in serological tests to diagnose Lyme disease. It is a key antigen in ELISA and Western blot assays, which detect antibodies against Borrelia in patient samples .
- Vaccine Development: OspC is a potential candidate for Lyme disease vaccines. Its role in early infection makes it a target for vaccine strategies aimed at preventing the establishment of infection.
- Research: Recombinant OspC is used in research to study the molecular interactions between Borrelia and the host immune system. It helps in understanding the mechanisms of immune evasion and pathogenesis.
