BOLA1 Human
BolA Homolog 1 Human Recombinant
Cat. No.
BT28185
Source
Escherichia Coli.
Synonyms
BolA-like protein 1, hBolA, BOLA1, CGI-143.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
BOLA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (21-137 a.a) and having a molecular mass of 14kDa.
BOLA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
BOLA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
BolA Homolog 1 (BOLA1), a member of the bolA/yrbA family, is a non-classical secreted protein. Studies have shown that BOLA1, along with other BolA proteins (hBolA2, hBolA3), can be secreted into the culture medium of transfected Cos-7 cells. This secretion process is independent of the Golgi apparatus and is not affected by BFA treatment.
Description
Recombinant Human BOLA1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein comprises 140 amino acids (residues 21-137) with a molecular weight of 14 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus. The purification process utilizes proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has undergone sterile filtration.
Formulation
The BOLA1 protein is supplied in a solution at a concentration of 1 mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and supplemented with 0.2M NaCl, 20% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (either HSA or BSA) at a concentration of 0.1%. It's important to avoid repeated freeze-thaw cycles.
Purity
The purity of this product is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
BolA-like protein 1, hBolA, BOLA1, CGI-143.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSQGSAGSG AIGPVEAAIR TKLEEALSPE VLELRNESGG HAVPPGSETH FRVAVVSSRF EGLSPLQRHR LVHAALAEEL GGPVHALAIQ ARTPAQWREN SQLDTSPPCL GGNKKTLGTP.
Product Science Overview
Structure and Characteristics
- Amino Acid Sequence: The recombinant human BOLA1 protein consists of 140 amino acids (21-137 a.a) and has a molecular mass of approximately 14 kDa .
- Tagging and Purification: BOLA1 is fused to a 23 amino acid His-tag at the N-terminus and is expressed in Escherichia coli (E. coli). The protein is then purified using conventional chromatography techniques .
- Purity: The recombinant protein is highly pure, with a purity greater than 95% as determined by SDS-PAGE .
Functional Insights
BolA proteins, including BOLA1, have been shown to be secreted into the culture medium of transfected Cos-7 cells, although they do not colocalize with the Golgi apparatus . Interestingly, the secretion of these proteins is not inhibited by Brefeldin A (BFA) treatment, which typically disrupts protein transport from the endoplasmic reticulum to the Golgi .
Applications and Usage
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