BMP 2 Human, HEK
Greater than 95.0% as determined by analysis by SDS-PAGE.
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Description
BMP-2 Human Recombinant produced in HEK cells is a glycosylated disulfide-linked homodimer, having a molecular weight range of 28kDa due to glycosylation. The BMP2 is purified by proprietary chromatographic techniques.
Product Specs
Recombinant Human BMP-2, produced in HEK cells, is a glycosylated homodimer with disulfide links. Its molecular weight, ranging around 28kDa, can vary due to glycosylation. The purification process involves proprietary chromatographic techniques.
The BMP2 was lyophilized from a solution of 0.67mg/ml in 2xPBS with 6% ethanol.
For reconstitution of lyophilized BMP-2, sterile 4mM HCl containing 0.1% endotoxin-free recombinant HSA is recommended.
Analysis by SDS-PAGE confirms a purity greater than 95.0%.
The specific activity, determined by the dose-dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line), was found to be 6.53ng/ml.
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Product Science Overview
BMP-2 is a low-molecular-weight glycoprotein that participates in several signaling pathways, including the hedgehog pathway, TGF-β signaling pathway, and cytokine-cytokine receptor interaction . It induces chondrocyte formation and osteoblast differentiation, which are essential processes for bone and cartilage growth . Additionally, BMP-2 is involved in embryo dorsal-ventral patterning and organogenesis .
Recombinant human BMP-2 (rhBMP-2) is produced using human embryonic kidney (HEK) cells. This recombinant form retains the biological activity of the native protein and is used extensively in clinical and research settings for bone tissue engineering and regenerative medicine . The use of HEK cells for producing rhBMP-2 ensures high purity and bioactivity, making it suitable for therapeutic applications.
rhBMP-2 has been widely used in orthopedic and dental surgeries to promote bone healing and regeneration. It is particularly effective in spinal fusion surgeries, where it helps in the formation of new bone tissue, reducing the need for bone grafts . Additionally, rhBMP-2 is used in the treatment of bone defects and non-unions, enhancing the body’s natural bone healing processes .
Research on BMP-2 continues to explore its potential in various medical applications. Studies have shown that BMP-2 can enhance the bioactivity of scaffolds used in bone tissue engineering, improving the efficiency of bone regeneration . Furthermore, BMP-2 has been implicated in adipogenesis, where it regulates the development of adipose tissue in a depot-specific manner .
