BMP6 Human

Bone Morphogenetic protein-6 Human Recombinant
Cat. No.
BT17179
Source
Escherichia Coli.
Synonyms
Bone morphogenetic protein 6, BMP-6, VG-1-related protein, VG-1-R, VGR-1, BMP6, VGR, VGR1.
Appearance
Sterile filtered colorless solution.
Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BMP6 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 164 amino acids (375-513) and having a molecular mass of 18kDa.
BMP6 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Bone morphogenetic proteins (BMPs), members of the transforming growth factor-beta (TGFB) superfamily, are secreted signaling molecules known for inducing ectopic bone growth. Initially discovered for their ability to stimulate endochondral osteogenesis in vivo outside the skeletal system, BMPs are crucial for bone development. Specifically, BMP6, expressed early in embryogenesis, is believed to play a role in early development. Its close relation to BMP5 and BMP7 suggests potential bone-inducing properties.
Description
Recombinant human BMP6, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 164 amino acids (375-513). With a molecular weight of 18 kDa, this protein comprises amino acids 375-513 of BMP6 and includes a 25 amino acid His-tag fused to its N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
BMP6 is supplied as a 0.25 mg/ml solution in 10 mM Sodium citrate buffer (pH 3.5) containing 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein like 0.1% HSA or BSA is advisable for long-term storage. Minimize repeated freeze-thaw cycles.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
Bone morphogenetic protein 6, BMP-6, VG-1-related protein, VG-1-R, VGR-1, BMP6, VGR, VGR1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSASSR RRQQSRNRST QSQDVARVSS ASDYNSSELK TACRKHELYV SFQDLGWQDW IIAPKGYAAN YCDGECSFPL NAHMNATNHA IVQTLVHLMN PEYVPKPCCA PTKLNAISVL YFDDNSNVIL KKYRNMVVRA CGCH.

Product Science Overview

Introduction

Bone Morphogenetic Protein-6 (BMP-6) is a member of the Transforming Growth Factor-beta (TGF-β) superfamily. This family of proteins is known for its role in regulating cell growth, differentiation, and development in various tissues. BMP-6, in particular, is recognized for its ability to induce the formation of bone and cartilage, making it a crucial factor in skeletal development and repair .

Gene and Protein Structure

The BMP-6 protein is encoded by the BMP6 gene located on chromosome 6 in humans. The protein consists of a signal peptide, a propeptide, and a mature peptide. The mature peptide is the active form of BMP-6 and is responsible for its biological functions. BMP-6 is synthesized as a precursor protein that undergoes proteolytic cleavage to release the mature, active form .

Biological Functions

BMP-6 plays a significant role in various biological processes, including:

  • Skeletal System Development: BMP-6 is essential for the development and maintenance of the skeletal system. It promotes the differentiation of mesenchymal stem cells into osteoblasts, which are responsible for bone formation .
  • Cartilage Formation: BMP-6 induces the formation of cartilage, a critical component of the skeletal system .
  • Cell Differentiation: BMP-6 influences the differentiation of various cell types, including chondrocytes (cartilage cells) and osteoblasts (bone cells) .
  • Regulation of Apoptosis: BMP-6 is involved in the regulation of programmed cell death, which is essential for maintaining tissue homeostasis .
Recombinant BMP-6

Recombinant human BMP-6 (rhBMP-6) is produced using recombinant DNA technology. This involves inserting the BMP6 gene into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. Recombinant BMP-6 is used in various research and clinical applications, including:

  • Bone Regeneration: rhBMP-6 is used to promote bone healing and regeneration in orthopedic and dental surgeries .
  • Tissue Engineering: rhBMP-6 is utilized in tissue engineering to develop bioengineered tissues for transplantation and repair .
  • Research: rhBMP-6 is used in scientific research to study its role in cell differentiation, bone formation, and other biological processes .
Clinical Applications

Recombinant BMP-6 has shown promise in several clinical applications, including:

  • Spinal Fusion: rhBMP-6 is used in spinal fusion surgeries to promote the fusion of vertebrae and improve patient outcomes .
  • Non-union Fractures: rhBMP-6 is used to treat non-union fractures, where the bone fails to heal properly .
  • Dental Implants: rhBMP-6 is used in dental implant procedures to enhance bone regeneration and improve implant stability .

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