BMP3 Human

Bone Morphogenetic protein-3 Human Recombinant
Cat. No.
BT16993
Source
Escherichia Coli.
Synonyms
Bone Morphogenetic Protein 3, Osteogenin, Bone Morphogenetic Protein 3 (Osteogenic), Bone Morphogenetic Protein 3A, BMP-3A, BMP-3, Bone Morphogenetic Protein-3, BMP3A, BMP3.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by: 
(a) Analysis by HPLC. 
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BMP3 Human Recombinant produced in E.coli is a non-glycosylated disulfide linked homodimer containing 2 chains of 110 amino acids and having a molecular mass of 24.8kDa.
The BMP-3 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Bone Morphogenetic Protein 3 (BMP3) belongs to the BMP family, with some members being part of the TGF-beta superfamily (BMP2-7). The BMP family consists of over 13 proteins involved in cartilage and bone formation, embryogenesis, and the morphogenesis of various tissues and organs. They regulate the growth, differentiation, chemotaxis, and apoptosis of diverse cell types. Similar to other TGF-beta family proteins, BMPs demonstrate high conservation across different animal species. For instance, mature human and rat BMP3 share a 98% amino acid sequence identity.
Description
Recombinant Human BMP3, produced in E.coli, is a non-glycosylated, disulfide-linked homodimer. It comprises two chains, each containing 110 amino acids, resulting in a molecular mass of 24.8kDa. The purification of BMP-3 is achieved using proprietary chromatographic methods.
Physical Appearance
The product appears as a sterile, filtered white powder that has been lyophilized (freeze-dried).
Formulation
The BMP-3 protein undergoes lyophilization from a 0.2µm filtered concentrated solution containing 30% Acetonitrile and 0.1% TFA.
Solubility
For reconstitution, it is advised to dissolve the lyophilized BMP3 in sterile 4mM HCl at a concentration not less than 100µg/ml. This solution can then be further diluted into other aqueous solutions as needed.
Stability
While the lyophilized BMP3 remains stable at room temperature for up to 3 weeks, it's recommended to store it desiccated below -18°C. After reconstitution, store BMP-3 at 4°C for 2-7 days. For long-term storage, keep it below -18°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for extended storage. Avoid freeze-thaw cycles.
Purity
The purity of the product exceeds 95.0%, as determined by: (a) High-Performance Liquid Chromatography (HPLC) analysis and (b) SDS-PAGE analysis.
Biological Activity
The ED50, representing the effective concentration at which the BMP3 exhibits 50% of its maximal activity, is determined by assessing its capacity to inhibit BMP-2-induced activity in murine MC3T3-E1 cells.
Synonyms
Bone Morphogenetic Protein 3, Osteogenin, Bone Morphogenetic Protein 3 (Osteogenic), Bone Morphogenetic Protein 3A, BMP-3A, BMP-3, Bone Morphogenetic Protein-3, BMP3A, BMP3.
Source
Escherichia Coli.
Amino Acid Sequence
QWIEPRNCAR RYLKVDFADI GWSEWIISPK SFDAYYCSGA CQFPMPKSLK PSNHATIQSI VRAVGVVPGI PEPCCVPEKM SSLSILFFDE NKNVVLKVYP NMTVESCACR.

Product Science Overview

Introduction

Bone Morphogenetic Protein-3 (BMP-3), also known as osteogenin, is a protein encoded by the BMP3 gene in humans. It is a member of the transforming growth factor-beta (TGF-β) superfamily, which plays a crucial role in various cellular processes, including growth, differentiation, and apoptosis . Unlike other bone morphogenetic proteins, BMP-3 inhibits the ability of other BMPs to induce bone and cartilage development, making it a unique regulator within this family .

Structure and Function

BMP-3 is a disulfide-linked homodimer, meaning it consists of two identical subunits connected by disulfide bonds . This protein negatively regulates bone density by antagonizing the differentiation of osteogenic progenitors, which are cells that have the potential to develop into bone-forming cells . BMP-3 achieves this by modulating the availability of TGF-β receptors to other ligands, thereby suppressing osteoblast differentiation .

Expression and Regulation

BMP-3 is highly expressed in fractured tissues, indicating its role in bone repair and regeneration . It is also hypermethylated in many cases of colorectal cancer, suggesting its potential as a biomarker for early-stage cancer detection . The expression of BMP-3 is regulated by various signaling pathways, including the SMAD-dependent and SMAD-independent pathways .

Recombinant BMP-3

Recombinant BMP-3 is produced using recombinant DNA technology, which involves inserting the BMP3 gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the large-scale production of BMP-3 for research and therapeutic purposes . Recombinant BMP-3 retains the biological activity of the native protein and can be used in various applications, including tissue engineering and regenerative medicine .

Applications and Implications

The unique inhibitory role of BMP-3 in bone formation makes it a valuable tool in studying bone biology and developing treatments for bone-related disorders. Its potential as a biomarker for colorectal cancer also opens up new avenues for early diagnosis and treatment . Additionally, recombinant BMP-3 can be used in tissue engineering to promote bone repair and regeneration in clinical settings .

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