BLVRB Human

Biliverdin Reductase B Human Recombinant
Cat. No.
BT17250
Source
Escherichia Coli.
Synonyms
FLR, BVRB, SDR43U1, MGC117413, BLVRB, Flavin reductase, FR, NADPH-dependent diaphorase, NADPH-flavin reductase, Biliverdin reductase B, BVR-B, Biliverdin-IX beta-reductase, Green heme-binding protein, GHBP.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BLVRB Human Recombinant amino produced in E.Coli is a single, non-glycosylated polypeptide chain containing 206 amino acids having a molecular mass of 22.1 kDa.
The BLVRB is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Biliverdin reductase B (BLVRB), also known as flavin reductase (FR) or NADH-dependent biliverdin reductase, is an enzyme primarily recognized for its role in heme catabolism. It catalyzes the conversion of biliverdin, a green pigment produced during heme breakdown, into bilirubin, a yellow pigment. This reaction involves the reduction of a double bond in biliverdin, facilitated by NADH or NADPH as electron donors. Beyond its function in bilirubin production, BLVRB exhibits broader reductase activity, capable of reducing various substrates including flavins, methylene blue, pyrroloquinoline quinone, riboflavin, and methemoglobin. While its precise cellular functions are still under investigation, BLVRB is implicated in cellular protection against oxidative stress and potentially in iron metabolism regulation. The enzyme is widely distributed across tissues, with particularly high expression in the liver, kidney, spleen, and brain, consistent with its roles in bilirubin metabolism and antioxidant defense.
Description
Recombinant human BLVRB, expressed in E. coli, is available as a purified, non-glycosylated polypeptide chain. This monomeric protein comprises 206 amino acids, resulting in a molecular mass of 22.1 kDa. The purification process employs proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Clear, colorless solution subjected to sterile filtration.
Formulation
The BLVRB protein is supplied in a buffer consisting of 20mM Tris-HCl (pH 8.5), 10% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To further enhance stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advisable. Repeated freeze-thaw cycles should be minimized.
Purity
The purity of BLVRB is determined to be greater than 95% using SDS-PAGE analysis.
Synonyms
FLR, BVRB, SDR43U1, MGC117413, BLVRB, Flavin reductase, FR, NADPH-dependent diaphorase, NADPH-flavin reductase, Biliverdin reductase B, BVR-B, Biliverdin-IX beta-reductase, Green heme-binding protein, GHBP.
Source
Escherichia Coli.
Amino Acid Sequence
MAVKKIAIFG ATGQTGLTTL AQAVQAGYEV TVLVRDSSRL PSEGPRPAHV VVGDVLQAAD VDKTVAGQDA VIVLLGTRND LSPTTVMSEG ARNIVAAMKA HGVDKVVACT SAFLLWDPTK VPPRLQAVTD DHIRMHKVLR ESGLKYVAVM PPHIGDQPLT GAYTVTLDGR GPSRVISKHD LGHFMLRCLT TDEYDGHSTY PSHQYQ.

Product Science Overview

Introduction

Biliverdin Reductase B (BLVRB) is a crucial enzyme in the heme metabolism pathway. It is responsible for converting biliverdin IXβ to bilirubin IXβ, which is a significant component of fetal bile . This enzyme plays a vital role in the clearance of heme and the regulation of oxidative stress in the body.

Gene and Structure

The BLVRB gene is located on chromosome 19, specifically in the region 19q13.13 to q13.2 . The gene encodes for the BLVRB protein, which is involved in various cellular responses to stimuli and nuclear receptor pathways . The structure of BLVRB includes a binding site for NADPH, which is essential for its reductase activity.

Function and Importance

BLVRB is involved in the reduction of biliverdin IXβ to bilirubin IXβ. This reaction is crucial for the detoxification of heme, a process that prevents the accumulation of potentially toxic heme and its derivatives in the body . Bilirubin, the product of this reaction, is a potent antioxidant that protects cells from oxidative damage.

Recombinant BLVRB

Recombinant BLVRB is produced using genetic engineering techniques to express the human BLVRB gene in a host organism, such as bacteria or yeast. This allows for the large-scale production of the enzyme for research and therapeutic purposes. Recombinant BLVRB retains the same functional properties as the naturally occurring enzyme, making it a valuable tool for studying heme metabolism and developing potential treatments for related disorders .

Clinical and Research Applications

The ability to produce recombinant BLVRB has significant implications for both clinical and research settings. In research, recombinant BLVRB is used to study the enzyme’s role in heme metabolism and its potential as a therapeutic target. Clinically, recombinant BLVRB could be used to develop treatments for conditions associated with heme metabolism disorders, such as jaundice and certain types of anemia .

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