BGN Mouse
Sf9, Insect cells.
BGN, DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A, Biglycan, Bone/cartilage proteoglycan I, Biglycan Proteoglycan, MRLS.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
BGN produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 574 amino acids (38-369 a.a.) and having a molecular mass of 64.6kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
BGN is expressed with a 242 hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Biglycan, abbreviated as BGN, is a small leucine-rich proteoglycan found in the extracellular matrix. Its primary function involves the assembly of collagen fibrils, which are essential structural components of various tissues. Additionally, BGN plays a significant role in muscle regeneration. The structure of BGN shares similarities with two other small proteoglycans, decorin and fibromodulin. Notably, BGN interacts with several proteins implicated in muscular dystrophy. These interactions include those with alpha-dystroglycan, alpha- and gamma-sarcoglycan, and collagen VI. Moreover, BGN is crucial for the proper assembly of the dystrophin-associated protein complex, which is vital for maintaining the structural integrity of muscle fibers.
Recombinant BGN, expressed in Sf9 insect cells, is a single, glycosylated polypeptide chain. This protein comprises 574 amino acids, specifically spanning from amino acid positions 38 to 369. It has a molecular mass of 64.6 kDa. However, on SDS-PAGE analysis, the apparent molecular size will appear between 50-70 kDa. The recombinant BGN is engineered to carry a 242 amino acid long hIgG-His-tag at its C-terminus. Purification of the protein is achieved using proprietary chromatographic techniques.
The product appears as a clear and colorless solution that has been sterilized by filtration.
The BGN solution is provided at a concentration of 0.5 mg/ml. It is formulated in Phosphate Buffered Saline (PBS) with a pH of 7.4 and contains 10% glycerol.
For short-term storage (2-4 weeks), the BGN solution should be kept at 4°C. For extended storage, it is recommended to store the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) to the solution before freezing can enhance its long-term stability. Repeated freeze-thaw cycles should be avoided.
The purity of the BGN protein is greater than 90%, as determined by SDS-PAGE analysis.
BGN, DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A, Biglycan, Bone/cartilage proteoglycan I, Biglycan Proteoglycan, MRLS.
Sf9, Insect cells.
ADPDEEASGS DTTSGVPDLD SVTPTFSAMC PFGCHCHLRV VQCSDLGLKT VPKEISPDTT LLDLQNNDIS ELRKDDFKGL QHLYALVLVN NKISKIHEKA FSPLRKLQKL YISKNHLVEI PPNLPSSLVE LRIHDNRIRK VPKGVFSGLR NMNCIEMGGN PLENSGFEPG AFDGLKLNYL
RISEAKLTGI PKDLPETLNE LHLDHNKIQA IELEDLLRYS KLYRLGLGHN QIRMIENGSL SFLPTLRELH LDNNKLSRVP AGLPDLKLLQ VVYLHSNNIT KVGINDFCPM GFGVKRAYYN GISLFNNPVP YWEVQPATFR CVTDRLAIQF GNYKKLEPKS CDKTHTCPPC PAPELLGGPS
VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.
Product Science Overview
Biglycan is composed of a core protein with leucine-rich repeats (LRRs) flanked by N-terminal and C-terminal cysteine-rich domains . The core protein is approximately 42 kDa in size and contains one or two glycosaminoglycan (GAG) side chains . These GAG chains are typically chondroitin sulfate or dermatan sulfate and are attached to the N-terminus of the core protein .
Biglycan is involved in various biological processes, including:
- Scaffolding Collagen Fibrils: It helps in organizing and stabilizing collagen fibrils within the ECM .
- Cell Signaling: Biglycan acts as a signaling molecule, interacting with receptors on the cell surface to regulate various cellular functions .
- Inflammation: It can act as a danger signal, stimulating proinflammatory signaling pathways and linking innate and adaptive immune responses .
Recombinant mouse biglycan is used in various research areas, including:
- Cell Growth Inhibition: It has been shown to inhibit the growth of mouse 3T3-L1 embryonic fibroblast adipose-like cells .
- Musculoskeletal System: Biglycan plays a crucial role in bone formation, muscle integrity, and synapse stability at the neuromuscular junction .
- Inflammatory Diseases: Due to its role in inflammation, biglycan is being studied as a potential therapeutic target for inflammatory diseases and skeletal muscular dystrophies .
