BHMT2 Human

Betaine-Homocysteine Methyltransferase 2 Human Recombinant
Cat. No.
BT3182
Source
Escherichia Coli.
Synonyms
BHMT2, Betaine--Homocysteine S-Methyltransferase 2, SMM-Hcy Methyltransferase, Betaine-Homocysteine Methyltransferase 2, S-Methylmethionine--Homocysteine S-Methyltransferase BHMT2, EC 2.1.1.10, EC 2.1.1.5.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BHMT2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 386 amino acids (1-363 a.a.) and having a molecular mass of 42.7kDa.
BHMT2 is fused to a 23 amino acid His-tag at N-terminus.

Product Specs

Introduction
Betaine-Homocysteine Methyltransferase 2 (BHMT2) is an enzyme that plays a crucial role in regulating homocysteine levels within the body. Homocysteine, a sulfur-containing amino acid, is essential for various methylation reactions. BHMT2 facilitates the conversion of homocysteine to methionine by transferring a methyl group from betaine. Methionine, in turn, acts as a methyl donor, participating in the methylation of DNA, proteins, lipids, and other vital cellular components. BHMT2 is one of two key enzymes capable of catalyzing this methyl transfer reaction. It utilizes S-methylmethionine (SMM) as the methyl donor in the conversion process. Dysregulation of homocysteine metabolism has been linked to various health issues, including cardiovascular diseases and neural tube defects such as spina bifida.
Description
Recombinant human BHMT2, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 386 amino acids (specifically, amino acids 1 to 363). With a molecular weight of 42.7 kDa, this BHMT2 variant features a 23-amino acid His-tag fused at its N-terminus.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The BHMT2 protein solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the BHMT2 solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (either HSA or BSA) at a concentration of 0.1% is advised for long-term storage. To maintain protein integrity, minimize repeated freeze-thaw cycles.
Purity
The purity of BHMT2 is determined using SDS-PAGE analysis and is consistently greater than 90.0%.
Synonyms
BHMT2, Betaine--Homocysteine S-Methyltransferase 2, SMM-Hcy Methyltransferase, Betaine-Homocysteine Methyltransferase 2, S-Methylmethionine--Homocysteine S-Methyltransferase BHMT2, EC 2.1.1.10, EC 2.1.1.5.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAPAGRP GAKKGILERL ESGEVVIGDG SFLITLEKRG YVKAGLWTPE AVIEHPDAVR QLHMEFLRAG SNVMQTFTFS ASEDNMESKW EDVNAAACDL AREVAGKGDA LVAGGICQTS IYKYQKDEAR IKKLFRQQLE VFAWKNVDFL IAEYFEHVEE AVWAVEVLKE SDRPVAVTMC IGPEGDMHDI TPGECAVRLV KAGASIVGVN CRFGPDTSLK TMELMKEGLE WAGLKAHLMV QPLGFHAPDC GKEGFVDLPE YPFGLESRVA TRWDIQKYAR EAYNLGVRYI GGCCGFEPYH IRAIAEELAP ERGFLPPASE KHGSWGSGLD MHTKPWIRAR ARREYWENLL PASGRPFCPS LSKPDF.

Product Science Overview

Structure and Function

BHMT-2 is highly homologous to Betaine-Homocysteine Methyltransferase (BHMT), but unlike BHMT, BHMT-2 cannot use betaine as a methyl donor . Instead, it specifically utilizes SMM, which is derived from plants and yeast . The enzyme is abundantly expressed in the liver and kidney, indicating its significant role in these organs .

Genetic and Evolutionary Aspects

The genes encoding BHMT and BHMT-2 are located adjacent to each other on human chromosome 5 (5q13), suggesting they are tandem duplicates . This proximity indicates a possible evolutionary relationship between the two enzymes. BHMT-2’s ability to use SMM instead of betaine might have evolved to provide an alternative pathway for homocysteine methylation in specific physiological conditions .

Clinical Relevance

BHMT-2’s role in homocysteine metabolism makes it a potential target for therapeutic interventions aimed at reducing homocysteine levels in the blood. Elevated homocysteine levels are associated with an increased risk of cardiovascular diseases, making BHMT-2 a significant enzyme in medical research .

Recombinant BHMT-2

Recombinant human BHMT-2 is produced using E. coli expression systems and is purified using conventional chromatography techniques . This recombinant form is used in various research applications to study the enzyme’s structure, function, and potential inhibitors .

Inhibitors and Activity

Several compounds have been tested as inhibitors of BHMT-2. Unlike BHMT, BHMT-2’s activity is not inhibited by dimethylglycine and betaine . Methionine is a stronger inhibitor of BHMT-2 compared to BHMT, and S-adenosylmethionine is a weak inhibitor of BHMT-2 . These differences in inhibitor sensitivity highlight the unique regulatory mechanisms of BHMT-2.

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BHMT Human
Betaine Homocysteine S-Methyltransferase Human Recombinant
Cat. No.
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Source
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