BD 3 Mouse
Beta Defensin-3 Mouse Recombinant
Cat. No.
BT13987
Source
Escherichia Coli.
Synonyms
Beta-defensin 3, BD-3, mBD-3, Defensin beta 3, Defb3, Bd3, MGC129397.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Beta Defensin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.6kDa.
The BD-3 is purified by proprietary chromatographic techniques.
The BD-3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Defensins are a family of peptides with microbicidal and cytotoxic properties produced by neutrophils. Sharing high protein sequence similarity, defensins, including beta-defensin 103A encoded by this gene, exhibit broad-spectrum antimicrobial activity and may play a crucial role in the innate immune defense of epithelial cells.
Description
Recombinant Mouse Beta Defensin-3, expressed in E.Coli, is a single, non-glycosylated polypeptide chain. It contains 41 amino acids, resulting in a molecular weight of 4.6kDa. The purification of BD-3 is carried out using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The Mouse BD-3 was lyophilized from a 0.2µm filtered solution in PBS, pH 7.4 at a concentration suitable for lyophilization.
Solubility
To reconstitute lyophilized Beta Defensin-3, it is recommended to dissolve it in sterile 18M-cm H2O to a concentration of at least 100µg/ml. This solution can then be further diluted into other aqueous solutions as needed.
Stability
Lyophilized Beta Defensin-3 Recombinant, while stable at room temperature for up to 3 weeks, should ideally be stored desiccated at a temperature below -18°C. Following reconstitution, BD-3 can be stored at 4°C for 2-7 days. For long-term storage, it should be kept at -18°C. Repeated freezing and thawing should be avoided.
Purity
Purity exceeding 98.0% as determined by:
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity is assessed based on its antimicrobial effect on E. coli. The effective concentration resulting in 50% inhibition (ED50) is typically observed within the range of 4-20µg/ml, which corresponds to a specific activity of 50,000-250,000 units/mg.
Synonyms
Beta-defensin 3, BD-3, mBD-3, Defensin beta 3, Defb3, Bd3, MGC129397.
Source
Escherichia Coli.
Amino Acid Sequence
KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K.
Product Science Overview
Structure and Characteristics
- Amino Acid Sequence: Mouse BD3 is a cysteine-rich, cationic peptide consisting of 41 amino acids .
- Molecular Mass: The predicted molecular mass of mouse BD3 is approximately 4.6 kDa .
- Disulfide Bonds: Beta defensins are characterized by their three intramolecular disulfide bonds, which distinguish them from alpha defensins .
Function and Mechanism
- Antimicrobial Activity: BD3 exhibits broad-spectrum antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses . It functions by disrupting the microbial cell membrane, leading to cell lysis and death .
- Role in Inflammation: BD3 is involved in the inflammatory response and is upregulated in response to infection and inflammation .
Recombinant Production
- Expression System: Recombinant mouse BD3 is typically produced in E. coli expression systems .
- Purity and Activity: The recombinant protein is purified to >95% purity and retains its antimicrobial activity, with an effective dose (ED50) for antimicrobial activity against E. coli being less than 20 µg/mL .
Applications
- Research: Recombinant BD3 is used in various research applications, including studies on antimicrobial mechanisms, immune response, and potential therapeutic uses .
- Therapeutic Potential: Due to its potent antimicrobial properties, BD3 is being explored as a potential therapeutic agent for treating infections and inflammatory diseases .
Storage and Stability
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