BD5 Human
Description
Product Specs
Product Science Overview
Beta Defensin-5 (hBD-5) is a member of the beta-defensin family, which are small cationic peptides known for their broad-spectrum antimicrobial properties. These peptides play a crucial role in the innate immune system by providing the first line of defense against pathogens. The recombinant form of Beta Defensin-5 is produced using advanced biotechnological methods, making it a valuable tool for research and potential therapeutic applications.
Beta Defensin-5 is a peptide consisting of 51 amino acids with a molecular mass of approximately 5.8 kDa . It is characterized by a six-cysteine motif that forms three intramolecular disulfide bonds, which are essential for its stability and function . The peptide is typically produced in Escherichia coli (E. coli) and purified using proprietary chromatographic techniques to achieve a purity greater than 95% .
Beta Defensin-5 exhibits antimicrobial activity by disrupting the membrane integrity of bacteria, fungi, and viruses. It achieves this through its cationic nature, which allows it to interact with the negatively charged microbial membranes, leading to membrane permeabilization and cell death . Additionally, Beta Defensin-5 can act as a chemoattractant for immature dendritic cells and memory T cells, thereby enhancing the immune response .
The recombinant form of Beta Defensin-5 is produced in E. coli and is available as a lyophilized (freeze-dried) powder . It is recommended to reconstitute the lyophilized peptide in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted as needed . The lyophilized form is stable at room temperature for up to three weeks, but for long-term storage, it should be kept desiccated below -18°C . Once reconstituted, the peptide should be stored at 4°C for short-term use and below -18°C for long-term use, with the addition of a carrier protein to prevent freeze-thaw cycles .
Beta Defensin-5 has promising therapeutic applications due to its antimicrobial properties. It is being studied for its potential use in treating drug-resistant infections, enhancing wound healing, and as an adjuvant in vaccines . Its ability to modulate the immune response also makes it a candidate for research in autoimmune diseases and cancer .
