BCL7C Human

B-cell CLL/lymphoma 7C Human Recombinant
Cat. No.
BT24998
Source
Escherichia Coli.
Synonyms
B-Cell CLL/Lymphoma 7C, B-Cell CLL/Lymphoma 7 Protein Family Member C, BCL7C.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

BCL7C Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 240 amino acids (1-217 a.a) and having a molecular mass of 25.9kDa (Molecular size on SDS-PAGE will appear higher).
BCL7C is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
B-cell CLL/lymphoma 7C (BCL7C), also known as BCL7C, shares similarities with BCL7A in its N-terminal region. Notably, BCL7A is directly implicated in a three-way gene translocation identified in a Burkitt lymphoma cell line. However, the function of the BCL7C gene remains to be elucidated.
Description
Recombinant BCL7C protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing 240 amino acids (specifically, residues 1-217). With a molecular weight of 25.9 kDa, it should be noted that its size on SDS-PAGE may appear larger. This discrepancy arises from the N-terminal 23 amino acid His-tag fused to BCL7C. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is provided as a sterile, colorless solution that has been filtered for sterility.
Formulation
The BCL7C protein solution has a concentration of 0.25 mg/ml and is formulated in a buffer composed of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended periods, storage at -20°C is recommended. To ensure optimal stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advised. Importantly, repeated freeze-thaw cycles should be avoided.
Purity
Analysis by SDS-PAGE confirms a purity exceeding 85%.
Synonyms
B-Cell CLL/Lymphoma 7C, B-Cell CLL/Lymphoma 7 Protein Family Member C, BCL7C.
Source
Escherichia Coli.

Product Science Overview

Gene and Protein Information
  • Gene Symbol: BCL7C
  • Protein Name: BAF Chromatin Remodeling Complex Subunit BCL7C
  • Aliases: BCL Tumor Suppressor 7C, B-Cell CLL/Lymphoma 7C .
Function and Pathways

The exact function of the BCL7C gene has not yet been fully determined. However, it is suggested that it may play an anti-apoptotic role . BCL7C is associated with several pathways, including:

  • MITF-M-dependent gene expression
  • Nervous system development .
Clinical Significance

Diseases associated with BCL7C include:

  • Lymphoma
  • Spondylolisthesis .
Recombinant Human BCL7C

Recombinant human BCL7C protein, fused to a His-tag at the N-terminus, is expressed in E. coli and purified using conventional chromatography techniques . This recombinant protein corresponds to the amino acids 1-217 of the human BCL7C .

Research and Applications

The recombinant BCL7C protein is used in various research applications, including:

  • Protein-protein interaction studies
  • Functional assays
  • Antibody production .
Storage and Handling

The recombinant human BCL7C protein should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain protein stability .

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