Introduction
B-cell CLL/lymphoma 7C (BCL7C), also known as BCL7C, shares similarities with BCL7A in its N-terminal region. Notably, BCL7A is directly implicated in a three-way gene translocation identified in a Burkitt lymphoma cell line. However, the function of the BCL7C gene remains to be elucidated.
Description
Recombinant BCL7C protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing 240 amino acids (specifically, residues 1-217). With a molecular weight of 25.9 kDa, it should be noted that its size on SDS-PAGE may appear larger. This discrepancy arises from the N-terminal 23 amino acid His-tag fused to BCL7C. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is provided as a sterile, colorless solution that has been filtered for sterility.
Formulation
The BCL7C protein solution has a concentration of 0.25 mg/ml and is formulated in a buffer composed of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended periods, storage at -20°C is recommended. To ensure optimal stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advised. Importantly, repeated freeze-thaw cycles should be avoided.
Purity
Analysis by SDS-PAGE confirms a purity exceeding 85%.
Synonyms
B-Cell CLL/Lymphoma 7C, B-Cell CLL/Lymphoma 7 Protein Family Member C, BCL7C.