Bcl 2 Human (1-206 a.a.)

B-Cell Leukemia/Lymphoma 2, (1-206 a.a.) Human Recombinant
Cat. No.
BT23252
Source
Escherichia Coli.
Synonyms
Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Bcl-2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-206. The wild type Bcl-2 is missing 12 amino acids from C-terminus. C-terminus is fused to His-Tag. C-terminus his-tag, mimics the deleted C-terminus membrane domain thus maintaining its biological activity. Bcl-2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
The BCL2 gene provides instructions for creating an integral outer mitochondrial membrane protein that prevents certain cells, like lymphocytes, from undergoing apoptosis (programmed cell death). When BCL2 is expressed constantly, for instance, due to its translocation to the Ig heavy chain locus, it is believed to contribute to the development of follicular lymphoma. Two transcript variants of this gene exist, resulting from alternative splicing, and they differ in their C-terminal ends.
Description
Bcl-2 Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of amino acids 1-206. The wild-type Bcl-2 lacks 12 amino acids at the C-terminus. A His-Tag is fused to the C-terminus, mimicking the deleted C-terminus membrane domain and preserving its biological activity. Bcl-2 is purified using proprietary chromatographic methods.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile.
Formulation
The protein is supplied in a buffer containing 10mM Tris-HCl (pH 8), 1mM EDTA, and 250mM NaCl.
Solubility
To reconstitute, suspend Bcl-2 in 100µl of 0.5M Acetic acid and leave overnight at 4°C. Dilute the solution 10-fold with your chosen buffer system. BCL-2 tends to form intramolecular disulfide bonds, so using 5mM DTT in the assay buffer is recommended. For SDS-PAGE, 10mM DTT is recommended.
Stability
Lyophilized Bcl-2 is stable at room temperature for up to 3 weeks, but it is best stored desiccated at a temperature below -18°C. After reconstitution, Bcl-2 should be stored at 4°C for 2-7 days. For longer storage, freeze at -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0%, as determined by: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis and (b) SDS-PAGE analysis.
Synonyms
Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
Source
Escherichia Coli.

Product Science Overview

Introduction

B-Cell Leukemia/Lymphoma 2 (BCL-2) is a protein encoded by the BCL2 gene in humans. It is a member of the BCL-2 family of regulator proteins that regulate cell death (apoptosis) by either inducing or inhibiting apoptosis. The BCL-2 protein is crucial in the regulation of the mitochondrial pathway of apoptosis, which is a key mechanism in the maintenance of cellular homeostasis and the prevention of cancer.

Structure and Function

The BCL-2 protein consists of several domains, including the BH1, BH2, BH3, and BH4 domains, which are involved in its anti-apoptotic function. The (1-206 a.a.) fragment of the BCL-2 protein represents the first 206 amino acids of the protein, which includes the BH4 domain and part of the BH3 domain. This fragment is significant because it retains the ability to inhibit apoptosis, making it a valuable tool for research and therapeutic applications.

Role in Cancer

BCL-2 is known for its role in cancer, particularly in B-cell malignancies such as B-cell leukemia and lymphoma. Overexpression of BCL-2 has been observed in various cancers, where it contributes to the resistance of cancer cells to apoptosis, allowing them to survive and proliferate uncontrollably. This makes BCL-2 a target for cancer therapy, with several BCL-2 inhibitors being developed and tested in clinical trials.

Human Recombinant BCL-2 (1-206 a.a.)

The human recombinant BCL-2 (1-206 a.a.) is a laboratory-produced version of the BCL-2 protein fragment. It is used in research to study the function of BCL-2 and its role in apoptosis and cancer. Recombinant proteins are produced using recombinant DNA technology, which involves inserting the gene encoding the protein into a host cell, such as bacteria or yeast, which then produces the protein.

Applications in Research and Medicine

The recombinant BCL-2 (1-206 a.a.) protein is used in various research applications, including:

  • Studying the Mechanisms of Apoptosis: Researchers use the recombinant protein to investigate how BCL-2 inhibits apoptosis and how this process is regulated.
  • Cancer Research: The protein is used to study the role of BCL-2 in cancer and to develop and test new cancer therapies targeting BCL-2.
  • Drug Development: The recombinant protein is used in the development of BCL-2 inhibitors, which are potential therapeutic agents for treating cancers that overexpress BCL-2.

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