BCCIP Human

BRCA2 And CDKN1A Interacting Protein Human Recombinant
Cat. No.
BT27304
Source
Escherichia Coli.
Synonyms
BRCA2 and CDKN1A-interacting protein, P21- and CDK-associated protein 1, Protein TOK-1, BCCIP, TOK1, BRCA2 and CDKN1A-interacting protein.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BCCIP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-314 a.a.) and having a molecular mass of 38.6kDa.
BCCIP is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
BCCIP (BRCA2 And CDKN1A Interacting Protein) is a nuclear protein with multiple conserved interacting domains. It plays a crucial role as a cofactor for BRCA2 in tumor suppression and regulates CDK2 kinase activity through its interaction with p21. BCCIP is also involved in the formation of BRCA2 and RAD51 nuclear foci, homologous recombination following double-strand breaks, and cell cycle progression.
Description
Recombinant human BCCIP, expressed in E. coli, is a non-glycosylated polypeptide chain containing 339 amino acids (residues 1-314) with a molecular weight of 38.6 kDa. The protein includes a 25-amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
BCCIP protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution is buffered with 20 mM Tris-HCl (pH 8.0) and contains 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the protein solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the BCCIP protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
BRCA2 and CDKN1A-interacting protein, P21- and CDK-associated protein 1, Protein TOK-1, BCCIP, TOK1, BRCA2 and CDKN1A-interacting protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMASRS KRRAVESGVP QPPDPPVQRD EEEEKEVENE DEDDDDSDKE KDEEDEVIDE EVNIEFEAYS LSDNDYDGIK KLLQQLFLKA PVNTAELTDL LIQQNHIGSV IKQTDVSEDS NDDMDEDEVF GFISLLNLTE RKGTQCVEQI QELVLRFCEK NCEKSMVEQL DKFLNDTTKP VGLLLSERFI NVPPQIALPM YQQLQKELAG AHRTNKPCGK CYFYLLISKT FVEAGKNNSK KKPSNKKKAA LMFANAEEEF FYEKAILKFN YSVQEESDTC LGGKWSFDDV PMTPLRTVML IPGDKMNEIM DKLKEYLSV.

Product Science Overview

Structure and Function

BCCIP is known to associate with BRCA2 and RAD51 during HR-mediated DNA repair. It is recruited to stalled replication forks and prevents the degradation of nascent DNA strands by the MRE11 nuclease . This function is essential for protecting the integrity of the genome, especially under conditions of replication stress, which can lead to genomic instability and oncogenic transformation if not properly managed .

Role in Replication Stress

Replication stress occurs when the progression of replication forks is hampered, leading to the formation of DNA lesions such as double-strand breaks (DSBs). BCCIP plays a pivotal role in stabilizing these stalled replication forks and preventing their collapse. In the absence of BCCIP, there is an increase in replication fork stalling and subsequent DNA double-strand break formation . This highlights the importance of BCCIP in maintaining the stability of the genome during DNA replication.

In Vivo Functions

Studies using mouse models have demonstrated the essential roles of BCCIP in embryonic development and chromosome stability. Conditional knockdown of BCCIP in mice leads to impaired cellular proliferation and increased apoptosis, resulting in embryonic lethality before day E11.5 . BCCIP-deficient mouse embryonic fibroblasts (MEFs) exhibit significant chromosomal structural alterations, including increased chromatid breaks and sister chromatid union (SCU), which can impair chromosome segregation during mitosis .

Implications for Cancer Research

Given its critical role in DNA repair and genomic stability, BCCIP is a protein of interest in cancer research. Deficiencies in BCCIP function can lead to increased genomic instability, a hallmark of cancer development. Understanding the mechanisms by which BCCIP operates can provide insights into potential therapeutic targets for cancer treatment.

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THE BIOTEK
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