BCAM Human
Sf9, Baculovirus cells.
Basal cell adhesion molecule isoform 1, BCAM, AU, CD239, LU, MSK19, Auberger B antigen, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein, CD_antigen: CD239, LU, MSK19.
Greater than 90% as determined by SDS-PAGE.
Description
BCAM produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 755 amino acids (32-547a.a.) and having a molecular mass of 83.2kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). BCAM is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Belonging to the immunoglobulin superfamily, Basal Cell Adhesion Molecule (BCAM), generated through alternate splicing of the Lutheran blood group molecule, comprises five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. Up-regulation of the BCAM protein is observed following malignant transformation of certain cell types both in vitro and in vivo. Moreover, BCAM interacts with integrin in sickle red blood cells and plays a role in vaso-occlusive episodes.
Produced in Sf9 Baculovirus cells, BCAM is a single, glycosylated polypeptide chain consisting of 755 amino acids (32-547a.a.) with a molecular mass of 83.2kDa. Note: SDS-PAGE analysis may show a molecular size of approximately 70-100kDa. The BCAM protein is expressed with a 239 amino acid hIgG-His tag at the C-Terminus and purified using proprietary chromatographic techniques.
The BCAM protein solution is provided at a concentration of 0.5mg/ml and contains Phosphate Buffered Saline (pH 7.4) along with 10% glycerol.
For optimal storage, refrigerate at 4°C if the entire vial will be used within 2-4 weeks. For extended storage periods, freeze at -20°C.
Adding a carrier protein (0.1% HSA or BSA) is recommended for long term storage.
Repeated freeze-thaw cycles should be avoided.
SDS-PAGE analysis indicates a purity greater than 90%.
Basal cell adhesion molecule isoform 1, BCAM, AU, CD239, LU, MSK19, Auberger B antigen, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein, CD_antigen: CD239, LU, MSK19.
Sf9, Baculovirus cells.
EVRLSVPPLV EVMRGKSVIL DCTPTGTHDH YMLEWFLTDR SGARPRLASA EMQGSELQVT MHDTRGRSPP YQLDSQGRLV LAEAQVGDER DYVCVVRAGA AGTAEATARL NVFAKPEATE VSPNKGTLSV MEDSAQEIAT CNSRNGNPAP KITWYRNGQR LEVPVEMNPE GYMTSRTVRE ASGLLSLTST LYLRLRKDDR DASFHCAAHY SLPEGRHGRL DSPTFHLTLH YPTEHVQFWV GSPSTPAGWV REGDTVQLLC RGDGSPSPEY TLFRLQDEQE EVLNVNLEGN LTLEGVTRGQ SGTYGCRVED YDAADDVQLS KTLELRVAYL DPLELSEGKV LSLPLNSSAV VNCSVHGLPT PALRWTKDST PLGDGPMLSL SSITFDSNGT YVCEASLPTV PVLSRTQNFT LLVQGSPELK TAEIEPKADG SWREGDEVTL ICSARGHPDP KLSWSQLGGS PAEPIPGRQG WVSSSLTLKV TSALSRDGIS CEASNPHGNK RHVFHFGTVS PQTSQAVEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH.
Product Science Overview
BCAM contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail . The protein functions as a receptor for the extracellular matrix protein, laminin . It is involved in various biological processes, including cell adhesion, cell-matrix adhesion, and signal transduction .
BCAM is widely expressed in various tissues, including the basal layer of epithelial cells and the endothelium of blood vessel walls . Its expression is higher in fetal tissues compared to adult tissues and is upregulated following malignant transformation in some cell types . Additionally, BCAM is expressed on erythrocytes, where it constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens .
BCAM has been implicated in several clinical conditions. It plays a role in epithelial cell cancer and in the vaso-occlusion of red blood cells in sickle cell disease . The interaction between BCAM and integrin in sickle red cells contributes to vasoocclusive episodes . Furthermore, BCAM has been shown to promote the metastasis of ovarian cancer .
Human recombinant BCAM is produced using recombinant DNA technology, which allows for the expression of the BCAM protein in a controlled laboratory environment. This recombinant form is used in various research applications to study the protein’s structure, function, and interactions.
