BAT1 Human

HLA-B Associated Transcript 1 Human Recombinant
Cat. No.
BT22834
Source
Escherichia Coli.
Synonyms
HLA-B associated transcript 1, UAP56, D6S81E, DDX39B, ATP-dependent RNA helicase p47, DEAD-box protein UAP56
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

BAT1 Human Recombinant protein produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 448 amino acids (1-428) and having a molecular mass of 51.1kDa. BAT1 is fused to 20 amino acid His Tag at N-terminus and is purified by proprietary chromatographic techniques.

Product Specs

Introduction
BAT1, a member of the DEAD box RNA-dependent ATPase family, plays a crucial role in pre-mRNA splicing by facilitating ATP hydrolysis. As an essential splicing factor, UAP56 protein is indispensable for the association of U2 small nuclear ribonucleoprotein with pre-mRNA and actively participates in mRNA export from the nucleus to the cytoplasm. Notably, mutations in this protein have been implicated in the development of rheumatoid arthritis.
Description
Recombinant BAT1 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 448 amino acids (residues 1-428). With a molecular weight of 51.1 kDa, it features a 20 amino acid His Tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The BAT1 protein solution (0.5 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability, consider adding a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing cycles should be avoided.
Purity
The purity of the protein is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
HLA-B associated transcript 1, UAP56, D6S81E, DDX39B, ATP-dependent RNA helicase p47, DEAD-box protein UAP56
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAENDVDNEL LDYEDDEVET AAGGDGAEAP AKKDVKGSYV SIHSSGFRDF LLKPELLRAI VDCGFEHPSE VQHECIPQAI LGMDVLCQAK SGMGKTAVFV LATLQQLEPV TGQVSVLVMC HTRELAFQIS KEYERFSKYM PNVKVAVFFG GLSIKKDEEV LKKNCPHIVV GTPGRILALA RNKSLNLKHI KHFILDECDK MLEQLDMRRD VQEIFRMTPH EKQVMMFSAT LSKEIRPVCR KFMQDPMEIF VDDETKLTLH GLQQYYVKLK DNEKNRKLFD LLDVLEFNQV VIFVKSVQRC IALAQLLVEQ NFPAIAIHRG MPQEERLSRY QQFKDFQRRI LVATNLFGRG MDIERVNIAF NYDMPEDSDT YLHRVARAGR FGTKGLAITF VSDENDAKIL NDVQDRFEVN ISELPDEIDI SSYIEQTR

Product Science Overview

Introduction

HLA-B Associated Transcript 1 (BAT1), also known as UAP56, D6S81E, DDX39B, ATP-dependent RNA helicase p47, and DEAD-box protein UAP56, is a protein encoded by the BAT1 gene in humans . This protein is part of the DEAD-box protein family, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). These proteins are implicated in various cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly .

Structure and Properties

The recombinant form of BAT1 is produced in E. coli and is a single, non-glycosylated polypeptide chain containing 448 amino acids, with a molecular mass of approximately 51.1 kDa . The predicted isoelectric point of BAT1 is 8.1 .

Function

BAT1 plays a crucial role in the regulation of mRNA splicing and export from the nucleus. It is involved in the assembly of the spliceosome, a complex responsible for removing introns from pre-mRNA. Additionally, BAT1 is essential for the nuclear export of mRNA, ensuring that processed mRNA is transported from the nucleus to the cytoplasm for translation .

Clinical Significance

BAT1 is associated with various diseases and conditions due to its role in RNA processing and immune response. It is located within the major histocompatibility complex (MHC) region on chromosome 6, which is known for its high polymorphism and association with immune-related diseases . The MHC region, also known as the human leukocyte antigen (HLA) region, is critical for antigen presentation and immune response .

Applications

Recombinant BAT1 is used in research to study its function in RNA processing and its role in diseases. It is also utilized in the development of therapeutic strategies targeting RNA splicing and export mechanisms.

Storage and Stability

For optimal stability, recombinant BAT1 should be stored at 2-8ºC and should avoid repeated freeze/thaw cycles . It is recommended to reconstitute the protein in 20mM Tris, 150mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL .

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