BASP1 Human

Brain Abundant Membrane Attached Signal Protein 1 Human Recombinant
Cat. No.
BT27046
Source
Escherichia Coli.
Synonyms
CAP-23, CAP23, NAP-22, NAP22, Brain acid soluble protein 1, BASP1, BASP1 Human, 22 kDa neuronal tissue-enriched acidic protein, Neuronal axonal membrane protein NAP-22.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BASP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 250 amino acids (1-227) and having a molecular mass of 25 kDa (Molecular size on SDS-PAGE will appear higher). BASP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Brain Abundant Membrane Attached Signal Protein 1 (BASP1) is a protein found in the membranes of cells. It has many places where it can be temporarily modified by a process called phosphorylation and also contains PEST motifs. The fact that PEST sequences are found in many different species suggests they are important for the protein's function. These sequences are often found in proteins that are broken down quickly by the cell. The ability of the immune system to recognize BASP1 is different depending on the species. BASP1 undergoes a modification called myristoylation at its N-terminal end.
Description
This product is a recombinant human BASP1 protein produced in E. coli bacteria. It is a single chain of 250 amino acids (specifically amino acids 1 through 227) that is not glycosylated, meaning it does not have sugars attached to it. This protein has a molecular weight of 25 kDa, although it may appear larger when analyzed by SDS-PAGE. This version of BASP1 has a 23 amino acid tag called a His-tag attached to its N-terminal end. This tag is used to purify the protein using specialized chromatography techniques.
Physical Appearance
A clear and colorless liquid that has been sterilized by filtration.
Formulation
The BASP1 protein is provided at a concentration of 0.25 mg per ml in a solution containing 20 mM Tris-HCl buffer at a pH of 8.0, 0.2 M NaCl (sodium chloride), and 20% glycerol.
Stability
If the entire vial will be used within 2-4 weeks, it can be stored at 4 degrees Celsius. For longer storage, it should be frozen at -20 degrees Celsius. To ensure the protein remains stable during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is recommended. Repeatedly freezing and thawing the protein should be avoided.
Purity
The purity of this protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
CAP-23, CAP23, NAP-22, NAP22, Brain acid soluble protein 1, BASP1, BASP1 Human, 22 kDa neuronal tissue-enriched acidic protein, Neuronal axonal membrane protein NAP-22.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGGKLSK KKKGYNVNDE KAKEKDKKAE GAATEEEGTP KESEPQAAAE PAEAKEGKEK PDQDAEGKAE EKEGEKDAAA AKEEAPKAEP EKTEGAAEAK AEPPKAPEQE QAAPGPAAGG EAPKAAEAAA APAESAAPAA GEEPSKEEGE PKKTEAPAAP AAQETKSDGA PASDSKPGSS EAAPSSKETP AATEAPSSTP KAQGPAASAE EPKPVEAPAA NSDQTVTVKE.

Product Science Overview

Gene and Protein Structure

The BASP1 gene is located on chromosome 5 and encodes a protein that consists of 227 amino acids . The protein has several key features:

  • Transient Phosphorylation Sites: These sites are crucial for the regulation of the protein’s activity.
  • PEST Motifs: These sequences are rich in proline (P), glutamic acid (E), serine (S), and threonine (T) and are typically found in proteins with high turnover rates .
  • N-terminal Myristoylation: This modification involves the addition of a myristoyl group to the N-terminal glycine residue, which is essential for membrane attachment .
Functional Significance

BASP1 is involved in several important cellular functions:

  • Neurite Outgrowth and Plasticity: BASP1 plays a key role in the development and remodeling of neurites, which are projections from the cell body of neurons .
  • Transcription Corepressor Activity: The protein has been shown to interact with other proteins to repress transcription, thereby regulating gene expression .
  • Signal Transduction: BASP1 is involved in various signaling pathways, including those mediated by Rho GTPases .
Expression and Localization

BASP1 is predominantly expressed in the brain, particularly in regions such as the hippocampus, amygdala, and cerebral cortex . It is also found in other tissues, including the testis and skeletal muscle . The protein is localized to the cell membrane, where it interacts with other membrane-bound proteins and participates in signal transduction .

Clinical Relevance

Alterations in BASP1 expression have been implicated in various neurological disorders. For instance, changes in BASP1 levels have been associated with neurodegenerative diseases and brain injuries . Additionally, BASP1 has been studied as a potential marker for neural stem cells in the neurogenic niches of the mammalian brain .

Research and Applications

Recombinant BASP1 is used in research to study its role in cellular processes and its potential therapeutic applications. The recombinant form of the protein is produced using genetic engineering techniques, allowing for the study of its structure and function in detail.

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