BSG Human
Sf9, Baculovirus cells.
Basigin isoform 2, 5F7, CD147, EMMPRIN, OK, TCSF, Leukocyte activation antigen M6, OK blood group antigen.
Greater than 90.0% as determined by SDS-PAGE.
Description
BSG produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 428 amino acids (22-207a.a.) and having a molecular mass of 47.5kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
BSG is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Belonging to the immunoglobulin superfamily, Basigin Isoform 2 (BSG) is a type 1 transmembrane protein characterized by N- and O-linked glycosylation. It interacts with various ligands, including integrins and cyclophilin proteins (Cyp-A and Cyp-B). BSG plays a critical role in intercellular recognition and participates in diverse immunological processes, cell differentiation, and development. Furthermore, it regulates multiple cellular functions, encompassing the production and release of the monocarboxylate transporter, spermatogenesis, and others. In the context of malignant cells, Basigin Isoform 2 contributes to invasion, metastasis, growth, and survival by promoting the production and release of matrix metalloproteinases by neighboring mesenchymal and tumor cells.
Produced in Sf9 Baculovirus cells, BSG is a single, glycosylated polypeptide chain comprising 428 amino acids (22-207a.a.) with a molecular mass of 47.5kDa. It's important to note that on SDS-PAGE, the molecular size will appear within the range of 40-57kDa. The protein is expressed with a 242 amino acid hIgG-His tag at the C-terminus and subsequently purified using proprietary chromatographic methods.
The BSG protein solution is provided at a concentration of 0.5mg/ml. It is formulated in a phosphate-buffered saline solution (pH 7.4) containing 10% glycerol.
Repeated freezing and thawing of the product should be avoided.
The purity of the BSG protein is determined to be greater than 90.0% based on SDS-PAGE analysis.
Basigin isoform 2, 5F7, CD147, EMMPRIN, OK, TCSF, Leukocyte activation antigen M6, OK blood group antigen.
Sf9, Baculovirus cells.
ADPAAGTVFT TVEDLGSKIL LTCSLNDSAT EVTGHRWLKG GVVLKEDALP GQKTEFKVDS DDQWGEYSCV FLPEPMGTAN IQLHGPPRVK AVKSSEHINE GETAMLVCKS ESVPPVTDWA WYKITDSEDK ALMNGSESRF FVSSSQGRSE LHIENLNMEA DPGQYRCNGT SSKGSDQAII
TLRVRSHLAL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG
QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH.
Product Science Overview
Basigin has multiple isoforms. The common form, known as basigin-2, contains two immunoglobulin domains, while the extended form, basigin-1, has three . The recombinant mature human CD147 consists of 195 amino acids and has a calculated molecular mass of 21.6 kDa. Due to glycosylation, the recombinant protein migrates as an approximately 30-40 kDa band in SDS-PAGE under reducing conditions .
Basigin is involved in numerous biological functions, including:
Recombinant human Basigin is produced using advanced biotechnological methods. The protein is expressed in suitable host cells, purified, and characterized to ensure its functionality and stability. This recombinant protein is widely used in research to study its various roles and interactions.
