B9D2 Human

The B9D2 gene is upregulated during mucociliary differentiation, a process where cells develop cilia to move mucus and other substances. The encoded protein localizes to basal bodies and cilia, essential components of the cellular structure . The recombinant human B9D2 protein is typically produced in E. coli and consists of a single, non-glycosylated polypeptide chain containing 198 amino acids (1-175) with a molecular mass of approximately 21.6 kDa .

B9D2 is involved in maintaining cilia stability and function. Disrupting the expression of this gene can lead to defects in cilia, which are critical for various cellular processes, including movement and signaling . The protein is also associated with basal bodies, which are the organizing centers for cilia and flagella .

Recombinant human B9D2 protein is widely used in research to study cilia-related functions and diseases. It is often tagged with a His-tag for easy purification and detection in experimental setups . The protein’s applications include:

• Antibody Competition Assays: Used as a blocking antigen to confirm antibody specificity .
• Functional Studies: Investigating the role of B9D2 in cilia formation and stability .
• Disease Research: Studying the implications of B9D2 dysfunction in ciliopathies, a group of disorders caused by cilia defects .

Recombinant B9D2 protein should be stored at 4°C for short-term use and at -20°C for long-term storage. It is recommended to avoid freeze-thaw cycles to maintain protein integrity. The protein is typically supplied in a buffer containing Tris-HCl, NaCl, glycerol, DTT, and EDTA .