B3GNT2 Human

Beta-1,3-N-Acetylglucosaminyltransferase 2 Human Recombinant
Cat. No.
BT3051
Source
Sf9, Baculovirus cells.
Synonyms
B3GNT2, B3GN-T2, B3GNT, B3GNT-2, B3GNT1, BETA3GNT, BGnT-2, BGNT2, N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2, Beta-1,3-N-acetylglucosaminyltransferase 1, BGnT-1, Beta-1,3-Gn-T1, Beta3Gn-T1, Beta-1,3-galactosyltransferase 7, Beta-1,3-GalTase 7, Beta3Gal-T7, Beta3GalT7, b3Gal-T7, Beta-3-Gx-T7, beta-GlcNAc beta-1,3-galactosyltransferase 7, betaGal beta-1,3-N-acetylglucosaminyltransferase 2, BGnT-2, Beta-1,3-Gn-T2, Beta-1,3-N-acetylglucosaminyltransferase 2, Beta3Gn-T2, beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

B3GNT2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 375 amino acids (29-397a.a.) and having a molecular mass of 43.5kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). B3GNT2 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2) is a member of the beta-1,3-N-acetylglucosaminyltransferase family involved in the synthesis of poly-N-acetyllactosamine. As a type II transmembrane protein, B3GNT2 exhibits a preference for the substrate lacto-N-neotetraose. It plays a crucial role in initiating and elongating poly-N-acetyllactosamine chains, acting as the primary polylactosamine synthase.
Description
Produced in Sf9 Baculovirus cells, B3GNT2 is a single, glycosylated polypeptide chain consisting of 375 amino acids (29-397a.a.) with a molecular mass of 43.5 kDa. Note that on SDS-PAGE, the apparent molecular size will be approximately 40-57 kDa. The protein is expressed with a C-terminal 6-amino acid His tag and purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, colorless solution.
Formulation
The B3GNT2 protein solution (0.5 mg/ml) is supplied in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
B3GNT2, B3GN-T2, B3GNT, B3GNT-2, B3GNT1, BETA3GNT, BGnT-2, BGNT2, N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2, Beta-1,3-N-acetylglucosaminyltransferase 1, BGnT-1, Beta-1,3-Gn-T1, Beta3Gn-T1, Beta-1,3-galactosyltransferase 7, Beta-1,3-GalTase 7, Beta3Gal-T7, Beta3GalT7, b3Gal-T7, Beta-3-Gx-T7, beta-GlcNAc beta-1,3-galactosyltransferase 7, betaGal beta-1,3-N-acetylglucosaminyltransferase 2, BGnT-2, Beta-1,3-Gn-T2, Beta-1,3-N-acetylglucosaminyltransferase 2, Beta3Gn-T2, beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPKSSSQEK NGKGEVIIPK EKFWKISTPP EAYWNREQEK LNRQYNPILS MLTNQTGEAG RLSNISHLNY CEPDLRVTSV VTGFNNLPDR FKDFLLYLRC RNYSLLIDQP DKCAKKPFLL LAIKSLTPHF ARRQAIRESW GQESNAGNQT VVRVFLLGQT PPEDNHPDLS DMLKFESEKH QDILMWNYRD TFFNLSLKEV LFLRWVSTSC PDTEFVFKGD DDVFVNTHHI LNYLNSLSKT KAKDLFIGDV IHNAGPHRDK KLKYYIPEVV YSGLYPPYAG GGGFLYSGHL ALRLYHITDQ VHLYPIDDVY TGMCLQKLGL VPEKHKGFRT FDIEEKNKNN ICSYVDLMLV HSRKPQEMID IWSQLQSAHL KCHHHHHH.

Product Science Overview

Structure and Function

B3GNT2 is involved in the glycosylation process, which is a common post-translational modification of proteins. Glycosylation involves the addition of sugar molecules to proteins, which can significantly alter their function and stability. B3GNT2 specifically catalyzes the transfer of N-acetylglucosamine (GlcNAc) to galactose residues, forming polylactosamine structures .

Polylactosamines are carbohydrate structures that are carried on N- and O-glycans as well as glycolipids. These structures are synthesized through the coordinated action of beta-1,4-galactosyltransferase (β4GalT) and beta-1,3-N-acetylglucosaminyltransferase (β3GnT). B3GNT2 exhibits intense activity toward oligosaccharide substrates possessing polylactosamine structures, suggesting that it is the principal polylactosamine synthase currently known .

Biological Importance

The glycosylation of proteins is essential for various biological functions. It is involved in protein folding, stability, and cell-cell interactions. B3GNT2, through its role in synthesizing polylactosamine chains, contributes to these processes. Polylactosamine structures are present on glycoproteins and glycolipids, which are involved in numerous cellular functions, including cell signaling and immune responses .

Clinical Relevance

Alterations in glycosylation patterns, including those mediated by B3GNT2, have been implicated in various diseases. For example, changes in glycosylation can affect tumor progression, immune responses, and the development of autoimmune diseases . Understanding the role of B3GNT2 in these processes can provide insights into disease mechanisms and potential therapeutic targets.

Recombinant B3GNT2

Recombinant B3GNT2 is produced using recombinant DNA technology, which involves inserting the gene encoding B3GNT2 into a suitable expression system, such as bacteria or mammalian cells. This allows for the production of large quantities of the enzyme for research and therapeutic purposes. Recombinant B3GNT2 retains the same enzymatic activity as the naturally occurring enzyme and is used in various biochemical and clinical studies .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.