ATG3 Antibody
Description
Product Specs
PAT15F6AT.
Anti-human ATG3mAb, clone PAT15F6AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ATG3 protein (1-314 amino acids purified from E. coli.
Mouse IgG1 k.
Product Science Overview
Autophagy is a crucial cellular process that involves the degradation and recycling of cellular components. It plays a vital role in maintaining cellular homeostasis and responding to various stress conditions. One of the key proteins involved in this process is Autophagy Related 3 (ATG3). This article provides a detailed background on ATG3, focusing on its role in autophagy, its molecular characteristics, and its significance in research, particularly with the use of mouse anti-human antibodies.
ATG3 is a ubiquitin-like-conjugating enzyme that is essential for the autophagy process. It is involved in the conjugation of ATG8-like proteins (such as LC3) to phosphatidylethanolamine (PE) on autophagic membranes . This conjugation is a critical step in the formation of autophagosomes, which are double-membrane structures that engulf cellular components for degradation .
ATG3 is encoded by the ATG3 gene, which is located on chromosome 3 in humans . The protein has several important domains that facilitate its function in autophagy. It interacts with ATG7, another ubiquitin-like-conjugating enzyme, to form a complex that is necessary for the lipidation of ATG8-like proteins . This interaction is crucial for the elongation and closure of autophagic membranes.
The study of ATG3 is significant in understanding the mechanisms of autophagy and its implications in various diseases. Dysregulation of autophagy has been linked to numerous conditions, including neurodegenerative diseases, cancers, and infectious diseases . By studying ATG3, researchers can gain insights into the molecular pathways involved in these diseases and potentially identify new therapeutic targets.
Mouse anti-human ATG3 antibodies are widely used in research to study the expression and function of ATG3 in human cells. These antibodies are generated by immunizing mice with human ATG3 protein, leading to the production of antibodies that specifically recognize human ATG3. These antibodies can be used in various applications, including Western blotting, immunofluorescence, and immunohistochemistry, to detect and quantify ATG3 in human samples .
