ATF4 Human

Activating Transcription Factor-4 Human Recombinant
Cat. No.
BT24975
Source
Escherichia Coli.
Synonyms
Cyclic AMP-dependent transcription factor ATF-4, cAMP-dependent transcription factor, ATF-4, Activating transcription factor 4, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, DNA-binding protein, TAXREB67, Tax-responsive enhancer element-binding protein 67, TaxREB67, ATF4, CREB2, TXREB.
Appearance
ATF4 is supplied as a sterile filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

ATF4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 510 amino acids (1-351 a.a.) and having a molecular mass of 56.6kDa.
ATF4 is fused to a 159 amino acid His-Calmodulin-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Activating transcription factor 4 (ATF4) is a DNA-binding protein belonging to a family that includes AP-1 transcription factors, cAMP-response element binding proteins, and CREB-like proteins. It was first discovered as a DNA binding protein found in mammals that could bind to a tax-responsive enhancer element in the LTR of HTLV-1.
Description
Recombinant human ATF4 protein, produced in E. coli, is a single, non-glycosylated polypeptide chain of 510 amino acids (amino acids 1-351). It has a molecular weight of 56.6 kDa. For purification purposes, the ATF4 protein is tagged at the N-terminus with a 159 amino acid His-Calmodulin tag.
Physical Appearance
ATF4 is a clear, sterile solution.
Formulation
ATF4 protein solution (0.5 mg/ml) in 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity determined by SDS-PAGE is greater than 90%.
Synonyms
Cyclic AMP-dependent transcription factor ATF-4, cAMP-dependent transcription factor, ATF-4, Activating transcription factor 4, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, DNA-binding protein, TAXREB67, Tax-responsive enhancer element-binding protein 67, TaxREB67, ATF4, CREB2, TXREB.
Source
Escherichia Coli.
Amino Acid Sequence
MHHHHHHMAD QLTEEQIAEF KEAFSLFDKD GDGTITTKEL GTVMRSLGQN PTEAELQDMI NEVDADGNGT IDFPEFLTMM ARKMKDTDSE EEIREAFRVF DKDGNGYISA AELRHVMTNL GEKLTDEEVD EMIREADIDG DGQVNYEEFV QMMTAKGSHM TEMSFLSSEV LVGDLMSPFD
QSGLGAEESL GLLDDYLEVA KHFKPHGFSS DKAKAGSSEW LAVDGLVSPS NNSKEDAFSG TDWMLEKMDL KEFDLDALLG IDDLETMPDD LLTTLDDTCD LFAPLVQETN KQPPQTVNPI GHLPESLTKP DQVAPFTFLQ PLPLSPGVLS STPDHSFSLE LGSEVDITEG DRKPDYTAYV
AMIPQCIKEE DTPSDNDSGI CMSPESYLGS PQHSPSTRGS PNRSLPSPGV LCGSARPKPY DPPGEKMVAA KVKGEKLDKK LKKMEQNKTA ATRYRQKKRA EQEALTGECK ELEKKNEALK ERADSLAKEI QYLKDLIEEV RKARGKKRVP.

Product Science Overview

Structure and Function

ATF4 is a basic leucine zipper (bZIP) transcription factor that binds to specific DNA sequences to regulate the transcription of target genes . It is involved in the unfolded protein response (UPR), a cellular stress response related to the ER . Under stress conditions, ATF4 is upregulated and activates genes that help the cell adapt and survive .

Role in Cellular Stress

ATF4 is a key player in the cellular stress response. It helps cells cope with ER stress by regulating the expression of genes involved in protein folding, antioxidant responses, and amino acid metabolism . In addition, ATF4 is involved in the regulation of ferroptosis, a form of programmed cell death characterized by iron-dependent lipid peroxidation . The dual role of ATF4 in promoting cell survival and ferroptosis highlights its complex function in cellular stress responses .

Involvement in Diseases

ATF4 has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic diseases . In cancer, ATF4 can either promote or inhibit tumor growth depending on the context . Its role in neurodegenerative diseases is linked to its function in managing oxidative stress and protein misfolding .

Research and Applications

Recent research has focused on understanding the genome-wide binding sites of ATF4 and its interaction with genetic variants associated with various traits and diseases . For example, studies have identified single-nucleotide polymorphisms (SNPs) that affect ATF4-mediated transcriptional activation, providing insights into the molecular mechanisms underlying these genetic associations .

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