ATF1 Human

ATF-1 is a protein coding gene that encodes a transcription factor. The human recombinant form of ATF-1 is produced in E. coli and is a single, non-glycosylated polypeptide chain containing 295 amino acids, with a molecular mass of approximately 31.8 kDa . The protein is fused to a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques .

ATF-1 influences cellular physiological processes by regulating the expression of genes related to growth, survival, and other cellular activities . It is particularly significant in the context of atherosclerosis, where it mediates the induction of heme oxygenase-1 (HO-1) by heme and drives macrophage adaptation to intraplaque hemorrhage . This process is crucial for protecting macrophages from foam cell formation and oxidative stress, which are key factors in the progression of atherosclerosis .

The human recombinant ATF-1 is used in various research applications, including studies on gene regulation and cellular responses to stress. It is typically stored at -20°C for long-term storage, with the recommendation to add a carrier protein to prevent multiple freeze-thaw cycles .