ASS1 Antibody

Argininosuccinate Synthase 1, Mouse Anti Human
Cat. No.
BT13980
Source
Synonyms
ASS, CTLN1, EC 6.3.4.5, ASS1, Argininosuccinate Synthase 1.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
ASS1 participates in the urea cycle, a series of reactions occurring within liver cells that process excess nitrogen generated from protein metabolism. This process converts the nitrogen into urea, which is then eliminated from the body through urine.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The solution contains 1mg/ml of ASS1 antibody in a buffer consisting of PBS at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
Store at 4°C for up to one month. For longer-term storage, keep at -20°C. Avoid repeated freezing and thawing cycles.
Stability / Shelf Life
The product remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The ASS1 antibody has undergone rigorous testing through ELISA, Western blot analysis, flow cytometry, and ICC/IF to ensure its specificity and reactivity. However, for optimal results, it is recommended to titrate the reagent for each specific application as requirements may vary.
Synonyms
ASS, CTLN1, EC 6.3.4.5, ASS1, Argininosuccinate Synthase 1.
Purification Method
ASS1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1G11AT.
Immunogen
Anti-human ASS1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ASS1 amino acids 1-412 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.

Product Science Overview

Mouse Anti-Human Antibodies

Mouse anti-human antibodies are monoclonal antibodies produced by immunizing mice with human antigens. These antibodies are highly specific and are commonly used in various immunological assays, including ELISA, Western blotting, and flow cytometry .

Production and Applications

Mouse anti-human antibodies are generated by immunizing mice with human immunoglobulins or other human proteins . The resulting antibodies are then harvested and purified using techniques such as immunoaffinity chromatography . These antibodies can be conjugated with various labels, such as horseradish peroxidase (HRP) or fluorescein isothiocyanate (FITC), to facilitate detection .

Specificity and Cross-Reactivity

Mouse anti-human antibodies are designed to recognize specific epitopes on human proteins, ensuring high specificity . They are often used as secondary antibodies in immunoassays to detect primary antibodies bound to their target antigens . These antibodies typically do not cross-react with immunoglobulins from other species, minimizing background noise and improving assay sensitivity .

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