Mouse anti-human antibodies are monoclonal antibodies produced by immunizing mice with human antigens. These antibodies are highly specific and are commonly used in various immunological assays, including ELISA, Western blotting, and flow cytometry .
Mouse anti-human antibodies are generated by immunizing mice with human immunoglobulins or other human proteins . The resulting antibodies are then harvested and purified using techniques such as immunoaffinity chromatography . These antibodies can be conjugated with various labels, such as horseradish peroxidase (HRP) or fluorescein isothiocyanate (FITC), to facilitate detection .
Mouse anti-human antibodies are designed to recognize specific epitopes on human proteins, ensuring high specificity . They are often used as secondary antibodies in immunoassays to detect primary antibodies bound to their target antigens . These antibodies typically do not cross-react with immunoglobulins from other species, minimizing background noise and improving assay sensitivity .