ASGR2 Human

Asialoglycoprotein Receptor 2 Human Recombinant
Cat. No.
BT25512
Source
Escherichia Coli.
Synonyms
Asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, C-type lectin domain family 4 member H2, Hepatic lectin H2, HL-2, ASGR2, CLEC4H2, HBXBP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

ASGR2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids (80-311 a.a) and having a molecular mass of 28.9kDa.
ASGR2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Asialoglycoprotein Receptor 2 (ASGR2) is a hetero-oligomeric protein composed of major and minor subunits, each encoded by distinct genes. Being the less abundant of the two, ASGR2 serves as a subunit within the asialoglycoprotein receptor complex. This transmembrane protein plays a critical role in maintaining serum glycoprotein balance. It achieves this by facilitating the endocytosis and subsequent lysosomal degradation of glycoproteins exhibiting exposed terminal galactose or N-acetylgalactosamine residues.
Description
Recombinantly produced in E. coli, the human ASGR2 protein is a single, non-glycosylated polypeptide chain encompassing 255 amino acids (specifically, residues 80-311). With a molecular weight of 28.9 kDa, this ASGR2 variant is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
This product is provided as a sterile, colorless solution, free from particulate matter.
Formulation
The ASGR2 protein solution is supplied at a concentration of 0.5 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 30% glycerol, and 1 mM DTT.
Stability
For optimal storage, the product should be kept at 4°C if it is to be used within 2-4 weeks. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of this protein is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms
Asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, C-type lectin domain family 4 member H2, Hepatic lectin H2, HL-2, ASGR2, CLEC4H2, HBXBP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSQSEGHRG AQLQAELRSL KEAFSNFSSS TLTEVQAIST HGGSVGDKIT SLGAKLEKQQ QDLKADHDAL LFHLKHFPVD LRFVACQMEL LHSNGSQRTC CPVNWVEHQG SCYWFSHSGK AWAEAEKYCQ LENAHLVVIN SWEEQKFIVQ HTNPFNTWIG LTDSDGSWKW VDGTDYRHNY KNWAVTQPDN WHGHELGGSE DCVEVQPDGR WNDDFCLQVY RWVCEKRRNA TGEVA.

Product Science Overview

Introduction

Asialoglycoprotein Receptor 2 (ASGR2) is a crucial component of the asialoglycoprotein receptor (ASGPR) system, primarily found in the liver. This receptor plays a significant role in the clearance of glycoproteins from the bloodstream. ASGR2, along with its counterpart ASGR1, forms a hetero-oligomeric complex that mediates the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues .

Structure and Function

ASGR2 is a transmembrane protein that is localized to the endoplasmic reticulum and plasma membrane . It is composed of a major 48 kDa subunit (ASGR1) and a minor 40 kDa subunit (ASGR2) . The receptor is responsible for recognizing and binding to desialylated glycoproteins, facilitating their internalization and subsequent degradation in lysosomes .

Biological Properties

ASGR2 is predominantly expressed in hepatocytes, the main functional cells of the liver . The receptor’s expression is regulated by various factors, including hormonal and nutritional states. It plays a vital role in maintaining serum glycoprotein homeostasis by removing glycoproteins that have lost their terminal sialic acid residues .

Clinical Significance

Mutations or dysregulation of ASGR2 can lead to various liver diseases, including hepatitis and autoimmune hepatitis . The receptor’s ability to clear glycoproteins from the bloodstream makes it a potential target for therapeutic interventions in liver-related disorders.

Research and Applications

Recent studies have identified splice variants of ASGR2, which may have different functional roles in hepatocytes . These variants could provide insights into the receptor’s diverse functions and its involvement in liver diseases. Recombinant forms of ASGR2 are used in research to study its structure, function, and potential therapeutic applications.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.