ASCC1 Human

Activating Signal Cointegrator 1 Complex Subunit 1 Human Recombinant

Cat. No.

BT25431

Source

Escherichia Coli.

Synonyms

Activating signal cointegrator 1 complex subunit 1, ASC1p50, CGI-18, p50, ASC-1 complex subunit p50, Trip4 complex subunit p50, ASCC1.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

ASCC1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 380 amino acids (1-357) and having a molecular mass of 43.6 kDa.
ASCC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

ASCC1 is a subunit of the triggering signal cointegrator 1 (ASC-1) complex, which acts as a transcriptional coactivator. The ASC-1 complex plays a crucial role in gene transactivation by interacting with various transcription factors, including activating protein 1 (AP-1), nuclear factor kappa-B (NF-kB), and serum response factor (SRF). ASCC1 possesses an N-terminal KH-type RNA-binding motif that is essential for AP-1 transactivation mediated by the ASC-1 complex. Notably, alterations in ASCC1 have been implicated in the development of Barrett's esophagus and esophageal adenocarcinoma.

Description

Recombinant human ASCC1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 380 amino acids (residues 1-357) with a molecular weight of 43.6 kDa. The protein includes a 23-amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless, and sterile-filtered solution.

Formulation

The ASCC1 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. To further enhance long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided.

Purity

The purity of the protein is determined to be greater than 90.0% as assessed by SDS-PAGE analysis.

Synonyms

Activating signal cointegrator 1 complex subunit 1, ASC1p50, CGI-18, p50, ASC-1 complex subunit p50, Trip4 complex subunit p50, ASCC1.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEVLRPQ LIRIDGRNYR KNPVQEQTYQ HEEDEEDFYQ GSMECADEPC DAYEVEQTPQ GFRSTLRAPS LLYKHIVGKR GDTRKKIEME TKTSISIPKP GQDGEIVITG QHRNGVISAR TRIDVLLDTF RRKQPFTHFL AFFLNEVEVQ EGFLRFQEEV LAKCSMDHGV DSSIFQNPKK LHLTIGMLVL LSEEEIQQTC EMLQQCKEEF INDISGGKPL EVEMAGIEYM NDDPGMVDVL YAKVHMKDGS NRLQELVDRV LERFQASGLI VKEWNSVKLH ATVMNTLFRK DPNAEGRYNL YTAEGKYIFK ERESFDGRNI LKLFENFYFG SLKLNSIHIS QRFTVDSFGN YASCGQIDFS.

Product Science Overview

Introduction

Activating Signal Cointegrator 1 Complex Subunit 1 (ASCC1) is a protein-coding gene that plays a crucial role in transcriptional regulation and DNA damage repair. The ASCC1 gene encodes a subunit of the Activating Signal Cointegrator 1 (ASC-1) complex, which is involved in gene transactivation by multiple transcription factors, including activating protein 1 (AP-1), nuclear factor kappa-B (NF-kB), and serum response factor (SRF).

Structure and Function

The ASCC1 protein contains an N-terminal KH-type RNA-binding motif, which is essential for its role in AP-1 transactivation by the ASC-1 complex. The ASC-1 complex is a tetrameric complex that includes other subunits such as TRIP4 (ASC1), ASCC2, and ASCC3. This complex can act as both a coactivator and a corepressor, depending on the context, and is involved in pre-mRNA processing and regulation of splicing.

Biological Significance

ASCC1 is involved in several critical biological pathways, including DNA damage reversal and homologous recombination repair (HRR) or single-strand annealing (SSA). It plays a role in the cellular response to DNA damage and is essential for maintaining genomic stability. Additionally, ASCC1 is implicated in the regulation of gene expression in response to gastrin-activated paracrine signals, particularly in the induction of SERPINB2 expression.

Clinical Relevance

Mutations in the ASCC1 gene are associated with several diseases, including Barrett esophagus and esophageal adenocarcinoma. Furthermore, ASCC1 is linked to spinal muscular atrophy with congenital bone fractures 2 (SMABF2), a rare genetic disorder characterized by severe muscle weakness and bone fractures.

Research and Applications

The human recombinant form of ASCC1 is used in various research applications to study its function and role in disease. Recombinant proteins are produced through genetic engineering techniques, allowing researchers to investigate the protein’s structure, function, and interactions in a controlled environment. This research is essential for developing potential therapeutic strategies targeting ASCC1-related pathways and diseases.

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ASCC1 Human

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