Artemin Human

Artemin is a member of the glial cell line-derived neurotrophic factor (GDNF) family, which is part of the larger transforming growth factor-beta (TGF-beta) superfamily of signaling molecules . This protein plays a crucial role in neurobiology due to its neurotrophic properties, which support the survival and growth of various neuron populations .

Artemin is synthesized as a preproprotein, which includes a signal sequence, a proregion, and a mature segment . The human recombinant form of Artemin is produced in Escherichia coli and is a non-glycosylated, disulfide-linked homodimer. Each polypeptide chain contains 113 amino acids, resulting in a total molecular mass of 24.2 kDa .

Artemin exerts its effects through the receptor complex composed of GFR-alpha 3 and the RET receptor . This signaling pathway is essential for the survival and maintenance of peripheral and central nervous system neurons. The interaction between Artemin and its receptors promotes neuron survival, differentiation, and neurite outgrowth .

Due to its neurotrophic properties, Artemin holds significant potential for therapeutic applications in neurodegenerative diseases and nerve injury . Research has shown that Artemin can support the survival of dopaminergic neurons, which are crucial in the context of Parkinson’s disease . Additionally, Artemin’s ability to promote neurite outgrowth makes it a promising candidate for nerve regeneration therapies .

Recombinant human Artemin is typically lyophilized after extensive dialysis against sodium citrate and sodium chloride . The lyophilized product is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it is recommended to store the solution at 4°C for short-term use and below -18°C for long-term use, with the addition of a carrier protein to prevent freeze-thaw cycles .