ART4 Human

ADP-Ribosyltransferase 4 Human Recombinant
Cat. No.
BT2603
Source

Sf9, Insect cells.

Synonyms

ARTC4, DOK1, CD297 Antigen, Dombrock Blood Group, ADP-Ribosyltransferase 4, Ecto-ADP-Ribosyltransferase 4.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 85% as determined by SDS-PAGE.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ART4 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 248 amino acids (47-285 a.a.) and having a molecular mass of 28.8kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). ART4 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ecto-ADP-Ribosyltransferase 4 (ART4) is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that carries Dombrock blood group antigens. It is primarily expressed in the fetal liver and its expression is regulated during erythroid differentiation. ART4 belongs to the Ribosyltransferase family.
Description
ART4, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain comprising 248 amino acids (residues 47-285) with a molecular weight of 28.8 kDa. It should be noted that on SDS-PAGE, the apparent molecular weight might range from 28 to 40 kDa. The protein is expressed with a 9-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The ART4 protein solution is provided at a concentration of 0.25 mg/ml in buffered saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the protein can be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of ART4 is determined to be greater than 85% by SDS-PAGE analysis.
Synonyms

ARTC4, DOK1, CD297 Antigen, Dombrock Blood Group, ADP-Ribosyltransferase 4, Ecto-ADP-Ribosyltransferase 4.

Source

Sf9, Insect cells.

Amino Acid Sequence

ADPSEVAIKI DFDFAPGSFD DQYQGCSKQV MEKLTQGDYF TKDIEAQKNY FRMWQKAHLA WLNQGKVLPQ NMTTTHAVAI LFYTLNSNVH SDFTRAMASV ARTPQQYERS FHFKYLHYYL TSAIQLLRKD SIMENGTLCY EVHYRTKDVH FNAYTGATIR FGQFLSTSLL KEEAQEFGNQ TLFTIFTCLG APVQYFSLKK EVLIPPYELF KVINMSYHPR GDWLQLRSTG NLSTYNCQLL KAHHHHHH.

Product Science Overview

Gene and Protein Structure

The ART4 gene encodes a protein that contains a mono-ADP-ribosylation (ART) motif. It is a single-copy gene in humans and has homologs in other primates and mice . The protein is glycosylphosphatidylinositol (GPI)-anchored to the erythrocyte membrane, which means it is attached to the cell membrane via a GPI anchor .

Expression and Function

ART4 is prominently expressed in the spleen and T-cells, with transcripts of 1.4, 2.4, and 5.5 kb detected in these tissues . Although enzymatic activity has not been demonstrated experimentally for ART4, it is believed to play a role in the immune response and cellular metabolism .

Clinical Significance

The Dombrock blood group system antigens are located on the ART4 gene product. Allelic variants of ART4 can lead to adverse transfusion reactions, making it clinically significant in blood transfusion medicine . Additionally, ART4 has been implicated in various cellular processes, including the regulation of cellular metabolism and the immune response .

Recombinant ART4

Recombinant ART4 proteins are produced using various expression systems, such as baculovirus, to study their structure and function. These recombinant proteins are often tagged with His-tags for purification and are used in research to understand the role of ART4 in cellular processes .

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