ARPP19 Human

CAMP-Regulated Phosphoprotein, 19kDa Human Recombinant
Cat. No.
BT25328
Source
Escherichia Coli.
Synonyms
CAMP-Regulated Phosphoprotein 19kDa, ARPP-19, ARPP16, Endosulfine Alpha-Like, ARPP-16, ENSAL, CAMP-Regulated Phosphoprotein 19, Cyclic AMP Phosphoprotein 19 KD, ARPP19.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ARPP19 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids (1-112 a.a) and having a molecular mass of 14.7kDa.
ARPP19 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
cAMP-regulated phosphoprotein 19 (ARPP19) acts as a protein phosphatase inhibitor, specifically targeting protein phosphatase 2A (PP2A) during the cell cycle's mitosis phase. Phosphorylation of ARPP19 at Serine-62 during mitosis enables it to bind with PPP2R2D (PR55-delta), inhibiting its activity. This interaction leads to the inactivation of PP2A, which is crucial for maintaining high cyclin-B1-CDK1 activity during the M phase. ARPP19 may also play a role in indirectly promoting GAP-43 expression. This 19 kDa cAMP-regulated phosphoprotein contributes to mitotic regulation by hindering protein phosphatase-2A.
Description
Recombinant human ARPP19 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 135 amino acids (with amino acids 1-112 being part of the ARPP19 sequence). It has a molecular weight of 14.7 kDa. This ARPP19 protein is produced with a 23 amino acid His-tag fused to its N-terminus and is purified using specialized chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
This ARPP19 protein solution has a concentration of 0.5mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep the vial refrigerated at 4°C. For extended storage, freeze the product at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage. Minimize repeated freeze-thaw cycles to preserve protein integrity.
Purity
Purity is assessed using SDS-PAGE and is determined to be greater than 90.0%.
Synonyms
CAMP-Regulated Phosphoprotein 19kDa, ARPP-19, ARPP16, Endosulfine Alpha-Like, ARPP-16, ENSAL, CAMP-Regulated Phosphoprotein 19, Cyclic AMP Phosphoprotein 19 KD, ARPP19.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSAEVPE AASAEEQKEM EDKVTSPEKA EEAKLKARYP HLGQKPGGSD FLRKRLQKGQ KYFDSGDYNM AKAKMKNKQL PTAAPDKTEV TGDHIPTPQD LPQRKPSLVA SKLAG.

Product Science Overview

Introduction

CAMP-Regulated Phosphoprotein, 19kDa (ARPP19) is a significant protein in cellular biology, particularly in the regulation of mitosis. This protein is encoded by the ARPP19 gene and is known for its role as a protein phosphatase inhibitor, specifically inhibiting protein phosphatase 2A (PP2A) during mitosis .

Structure and Function

ARPP19 is a 19-kilodalton protein that consists of 135 amino acids. It is a non-glycosylated polypeptide chain produced in E. coli for recombinant purposes . The protein’s primary function is to regulate mitosis by inhibiting PP2A, a critical enzyme in cell cycle control. By inhibiting PP2A, ARPP19 ensures proper progression through mitosis, preventing premature dephosphorylation of mitotic substrates .

Biological Significance

The inhibition of PP2A by ARPP19 is crucial for maintaining the fidelity of cell division. PP2A is involved in various cellular processes, including cell growth, division, and signaling. During mitosis, the precise regulation of PP2A activity by ARPP19 is essential for the correct segregation of chromosomes and the prevention of aneuploidy, which can lead to cancer and other diseases .

Recombinant Production

Recombinant ARPP19 is produced using bacterial expression systems, typically E. coli. The recombinant protein is purified to ensure it is free from contaminants and retains its biological activity. This recombinant form is used in research to study the protein’s function and its role in cellular processes .

Applications in Research

ARPP19 is used in various research applications, including:

  • Cell Cycle Studies: Understanding the regulation of mitosis and the role of PP2A inhibition.
  • Cancer Research: Investigating the implications of ARPP19 and PP2A in cancer development and progression.
  • Drug Development: Exploring potential therapeutic targets involving ARPP19 and PP2A for cancer and other diseases .

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