ARPC3 Human

Actin Related Protein 2/3 Complex, Subunit 3 Human Recombinant
Cat. No.
BT12419
Source
E.coli.
Synonyms
ARC21, p21-Arc, Actin-related protein 2/3 complex subunit 3, Arp2/3 complex 21 kDa subunit, ARPC3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ARPC3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-178 a.a) and having a molecular mass of 22.9kDa. ARPC3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Actin-related protein 2/3 complex subunit 3 (ARPC3), a member of the ARPC3 family, constitutes one of the seven subunits within the human Arp2/3 protein complex. This complex plays a crucial role in regulating actin polymerization. In conjunction with an activating nucleation-promoting factor (NPF), it mediates the formation of branched actin networks. ARPC3, primarily found in the cytoplasm and cytoskeleton, interacts with p20-ARC and contributes to the structural integrity of the protein complex.
Description
Recombinant human ARPC3, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 201 amino acids (specifically, amino acids 1 to 178). It possesses a molecular mass of 22.9 kDa. ARPC3 is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized through filtration.
Formulation
ARPC3 protein solution at a concentration of 0.5 mg/ml. The solution is buffered with 20 mM Tris-HCl at a pH of 8.0 and contains 0.15 M NaCl, 30% glycerol, and 1 mM DTT.
Stability
For optimal storage, keep the protein at 4°C if the entire vial will be used within 2-4 weeks. For longer-term storage, freeze the protein at -20°C. When storing for extended periods, it is advisable to add a carrier protein such as 0.1% HSA or BSA. Repeated freezing and thawing of the protein should be avoided.
Purity
The purity is determined to be greater than 95% based on SDS-PAGE analysis.
Synonyms
ARC21, p21-Arc, Actin-related protein 2/3 complex subunit 3, Arp2/3 complex 21 kDa subunit, ARPC3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPAYHSS LMDPDTKLIG NMALLPIRSQ FKGPAPRETK DTDIVDEAIY YFKANVFFKN YEIKNEADRT LIYITLYISE CLKKLQKCNS KSQGEKEMYT LGITNFPIPG EPGFPLNAIY AKPANKQEDE VMRAYLQQLR QETGLRLCEK VFDPQNDKPS KWWTCFVKRQ FMNKSLSGPG Q.

Product Science Overview

Introduction

Actin Related Protein 2/3 Complex, Subunit 3 (ARPC3) is a crucial component of the Arp2/3 complex, a seven-subunit protein complex that plays a significant role in the regulation of the actin cytoskeleton. The ARPC3 gene encodes one of these seven subunits and is highly conserved through evolution .

Structure and Function

The Arp2/3 complex is composed of two actin-related proteins, ARP2 and ARP3, along with five additional subunits, including ARPC3. This complex is essential for actin polymerization, a process that is critical for various cellular functions such as cell motility, phagocytosis, and intracellular transport of vesicles .

The ARPC3 subunit, specifically, is involved in the formation of branched actin networks. It binds to the sides of existing actin filaments (referred to as “mother” filaments) and initiates the growth of new filaments (referred to as “daughter” filaments) at a distinctive 70-degree angle. This branching mechanism is vital for creating the dynamic and complex actin cytoskeleton structures necessary for cellular processes .

Evolutionary Conservation

The Arp2/3 complex, including the ARPC3 subunit, has been conserved through evolution and is found in most eukaryotic organisms. However, it is absent in certain groups such as Chromalveolates and plants . This conservation underscores the fundamental role of the Arp2/3 complex in cellular biology.

Mechanism of Action

The Arp2/3 complex is activated by nucleation-promoting factors (NPFs), such as members of the Wiskott-Aldrich syndrome protein (WASP) family. These NPFs interact with actin monomers and the Arp2/3 complex to create a nucleation core, which serves as the starting point for new actin filament growth. The ARPC3 subunit, along with the other components of the Arp2/3 complex, facilitates this process by binding to pre-existing filaments and promoting the polymerization of new actin filaments .

Biological Significance

The regulation of actin polymerization by the Arp2/3 complex is crucial for various cellular activities. In the cytoplasm, the complex provides the force required for cell motility and other dynamic processes. Additionally, the Arp2/3 complex has been implicated in nuclear functions, such as gene transcription and DNA repair, by promoting actin polymerization within the nucleus .

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