ARG1 Human

Arginase-1 Human Recombinant
Cat. No.
BT22390
Source
Escherichia Coli.
Synonyms
EC 3.5.3.1, Arginase 1, Type I Arginase, Liver-Type Arginase.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ARG1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 330 amino acids (1-322a.a.) and having a molecular mass of 35.8kDa. ARG1 protein is fused to an 8 amino acid His tag at C-terminus and is purified by standard chromatography.

Product Specs

Introduction
ARG1 is an enzyme that breaks down arginine into ornithine and urea. There are two forms of arginase in mammals, each with different locations, functions, and characteristics. ARG1 is found in the cytoplasm of cells, primarily liver cells, where it participates in the urea cycle. A deficiency in ARG1, inherited in an autosomal recessive manner, leads to argininemia, a disorder marked by elevated ammonia levels in the blood.
Description
Recombinant human ARG1 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 330 amino acids (specifically, amino acids 1 through 322 with an 8 amino acid His tag fused at the C-terminus). This protein has a molecular weight of 35.8 kDa and is purified using standard chromatography techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The ARG1 human protein solution is provided at a concentration of 0.5 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8), 2 mM DTT, 0.1 M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 85% based on SDS-PAGE analysis.
Synonyms
EC 3.5.3.1, Arginase 1, Type I Arginase, Liver-Type Arginase.
Source
Escherichia Coli.
Amino Acid Sequence
MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PKLEHHHHHH.

Product Science Overview

Structure and Function

Arginase-1 is abundantly expressed in the liver but is also found in other tissues and cells that lack a complete urea cycle, such as the lungs . The enzyme’s primary function is to detoxify ammonia by converting it into urea, which is then excreted from the body. This process is vital for maintaining the body’s nitrogen balance.

Recombinant Human Arginase-1

Recombinant human arginase-1 (rhArg1) is a form of the enzyme that has been produced using recombinant DNA technology. This technology allows for the production of large quantities of the enzyme, which can be used for various research and therapeutic purposes.

Therapeutic Applications

One of the most promising applications of recombinant human arginase-1 is in cancer therapy. Many cancer cells are auxotrophic for arginine, meaning they cannot synthesize this amino acid and rely on external sources. By depleting arginine, rhArg1 can inhibit the growth of these cancer cells, leading to tumor regression . This approach is being developed as a broad-spectrum anti-cancer therapy, with various rhArg1 variants showing remarkable anti-cancer activity .

Clinical Studies

Clinical studies have been conducted to evaluate the safety, pharmacokinetics, and potential anti-tumor activity of pegylated recombinant human arginase-1 (peg-rhArg1) in patients with advanced hepatocellular carcinoma (HCC). These studies have shown that peg-rhArg1 has a manageable safety profile and preliminary evidence of activity in advanced HCC patients .

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THE BIOTEK
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