ARFIP2 Human

ADP-Ribosylation Factor Interacting Protein 2 Human Recombinant
Cat. No.
BT13441
Source
E.coli.
Synonyms
ADP-ribosylation factor interacting protein 2, partner of RAC1 (arfaptin 2), Partner of RAC1, Protein POR1, arfaptin-2, POR1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ARFIP2 Human Recombinant produced in E. coli is a single polypeptide chain containing 364 amino acids (1-341) and having a molecular mass of 40.2 kDa.
ARFIP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Arfaptin 2 (ARFIP2) plays a crucial role in Rac1-mediated actin polymerization by binding to Rac1. This interaction is vital for the formation of membrane ruffles and lamellipodia. As a potential target protein of ADP-ribosylation factor, ARFIP2 is implicated in membrane ruffling and exhibits elevated expression at neurodegeneration sites. Additionally, ARFIP2 interacts with ADP ribosylation factor 6 (ARF6), a GTPase associated with the plasma membrane and intracellular endosome vesicles, in a GTP-dependent manner. It has also been observed to regulate the aggregation of mutant Huntingtin protein by influencing proteasome function.
Description
Recombinant Human ARFIP2, expressed in E. coli, is a single polypeptide chain with a molecular weight of 40.2 kDa. It consists of 364 amino acids, spanning from position 1 to 341. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ARFIP2 solution is provided at a concentration of 0.25mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 1mM DTT, and 40% glycerol.
Stability
For optimal storage, the ARFIP2 solution should be kept at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. It is important to avoid repeated cycles of freezing and thawing.
Purity
Analysis by SDS-PAGE indicates a purity greater than 90%.
Synonyms
ADP-ribosylation factor interacting protein 2, partner of RAC1 (arfaptin 2), Partner of RAC1, Protein POR1, arfaptin-2, POR1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGS TDGILG KAATMEIPIH GNGEARQLPE DDGLEQDLQQ VMVSGPNLNE TSIVSGGYGG SGDGLIPTGS GRHPSHSTTP SGPGDEVARG IAGEKFDIVK KWGINTYKCT KQLLSERFGR GSRTVDLELE LQIELLRETK RKYESVLQLG RALTAHLYSL LQTQHALGDA FADLSQKSPE LQEEFGYNAE TQKLLCKNGE TLLGAVNFFV SSINTLVTKT MEDTLMTVKQ YEAARLEYDA YRTDLEELSL GPRDAGTRGR LESAQATFQA HRDKYEKLRG DVAIKLKFLE ENKIKVMHKQ LLLFHNAVSA YFAGNQKQLE QTLQQFNIKL RPPGAEKPSW LEEQ

Product Science Overview

Gene and Protein Structure

The ARFIP2 gene is located on chromosome 11 and encodes a protein that is involved in several cellular functions. The protein is known for its ability to bind to GTP-dependent proteins and small GTPases, which are essential for intracellular signaling and transport .

Functions and Mechanisms

  1. Actin Cytoskeleton Organization: ARFIP2 is involved in the regulation of the actin cytoskeleton, which is crucial for maintaining cell shape, enabling cell movement, and facilitating intracellular transport. It interacts with Rac1, a small GTPase, to mediate actin polymerization and the formation of membrane ruffles and lamellipodia .

  2. Vesicle-Mediated Transport: ARFIP2 plays a role in the secretion of metalloproteinases (MMPs) from the trans-Golgi network. This process is vital for various cellular activities, including tissue remodeling and repair .

  3. Autophagy and Mitophagy: ARFIP2 is a regulator of autophagy and mitophagy, processes that are essential for cellular homeostasis. It regulates the trafficking of ATG9A vesicles, which are involved in the formation of autophagosomes. This function is particularly important in podocytes, specialized cells in the kidneys that are vulnerable to oxidative stress .

  4. NF-kappa-B Inhibition: ARFIP2 also plays a role in inhibiting the NF-kappa-B signaling pathway by interacting with IKBKB and IKBKG. This pathway is involved in inflammatory responses and immune regulation .

Clinical Significance

Mutations or dysregulation of the ARFIP2 gene have been associated with various diseases, including myoma and familial hypertrophic cardiomyopathy. Additionally, ARFIP2’s role in autophagy and mitophagy makes it a potential target for therapeutic interventions in diseases characterized by oxidative stress and cellular damage, such as diabetic nephropathy .

Research and Applications

Recombinant human ARFIP2 is used in research to study its functions and mechanisms in detail. It is also employed in various assays to understand its role in cellular processes and its potential as a therapeutic target.

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