ARFIP1 341 a.a. Human
ADP-Ribosylation Factor Interacting Protein 1 341 a.a Human Recombinant
Cat. No.
BT13279
Source
Escherichia Coli.
Synonyms
HSU52521, ADP-ribosylation factor-interacting protein 1, Arfaptin-1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ARFIP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 364 amino acids (1-341 a.a) and having a molecular mass of 41.0kDa.
ARFIP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
ARFIP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1), containing one AH domain, is believed to be a target protein of the ADP-ribosylation factor.
Description
Recombinant human ARFIP1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 364 amino acids (specifically, amino acids 1 through 341) and has a molecular weight of 41.0 kDa. This protein includes a 23 amino acid His-tag located at the N-terminus. Purification is carried out using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The ARFIP1 protein solution is provided at a concentration of 0.25 mg/ml and contains phosphate buffered saline (pH 7.4), 30% glycerol, and 1mM DTT.
Stability
For optimal storage, maintain the product at 4°C if the entire vial will be used within 2-4 weeks. For extended storage periods, store the product frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It's crucial to avoid subjecting the product to multiple freeze-thaw cycles.
Purity
SDS-PAGE analysis has determined a purity exceeding 90.0%.
Synonyms
HSU52521, ADP-ribosylation factor-interacting protein 1, Arfaptin-1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQESPK NSAAEIPVTS NGEVDDSREH SFNRDLKHSL PSGLGLSETQ
ITSHGFDNTK EGVIEAGAFQ GGQRTQTKSG PVILADEIKN PAMEKLELVR KWSLNTYKCT RQIISEKLGR
GSRTVDLELE AQIDILRDNK KKYENILKLA QTLSTQLFQM VHTQRQLGDA FADLSLKSLE LHEEFGYNAD
TQKLLAKNGE TLLGAINFFI ASVNTLVNKT IEDTLMTVKQ YESARIEYDA YRTDLEELNL GPRDANTLPK
IEQSQHLFQA HKEKYDKMRN DVSVKLKFLE ENKVKVLHNQ LVLFHNAIAA YFAGNQKQLE QTLKQFHIKL
KTPGVDAPSW LEEQ.
Product Science Overview
Structure and Characteristics
Functional Role
ARFIP1 is involved in several key cellular processes:
- Phosphatidylinositol-4-phosphate Binding: ARFIP1 has the ability to bind to phosphatidylinositol-4-phosphate, a phospholipid that plays a significant role in membrane trafficking .
- Regulation of Protein Secretion: It is implicated in the regulation of protein secretion and intracellular protein transport. Specifically, ARFIP1 is involved in the negative regulation of retrograde transport from endosomes to the Golgi apparatus .
- Secretory Granule Formation: ARFIP1 plays a role in the biogenesis of secretory granules at the trans-Golgi network. It binds to ARF-GTP at the neck of a growing secretory granule precursor, forming a protective scaffold. Once the granule precursor is fully loaded, ARFIP1 is phosphorylated by PRKD1, leading to its release and subsequent granule fission .
Clinical and Research Applications
The recombinant form of ARFIP1 is used extensively in laboratory research to study its function and role in cellular processes. It is also used in various assays and experiments to understand its interaction with other proteins and its involvement in diseases related to membrane trafficking and protein secretion .
Storage and Stability
The recombinant ARFIP1 protein is typically stored in phosphate-buffered saline (pH 7.4) with 20% glycerol and 1mM DTT. It is recommended to store the protein at 4°C if it will be used within 2-4 weeks, or at -20°C for longer periods. To ensure stability, it is advised to avoid multiple freeze-thaw cycles .
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