ARC Human
Activity-Regulated Cytoskeleton-Associated Protein Human Recombinant
Cat. No.
BT25022
Source
E.coli.
Synonyms
Arg3.1, KIAA0278, Activity-regulated cytoskeleton-associated protein, Activity-regulated gene 3.1 protein homolog, ARC.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ARC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 416 amino acids (1-396 a.a.) and having a molecular mass of 47.4kDa. ARC is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
The Activity-regulated cytoskeleton-associated protein (ARC) plays a crucial role in regulating cell morphology and the organization of the cytoskeleton. This protein is essential for consolidating synaptic plasticity, a process fundamental for learning and memory formation. Additionally, ARC is involved in the formation of long-term memories. It regulates the process of AMPA receptor endocytosis, which is triggered by synaptic activity. This process is vital for regulating the strength of synaptic connections. Moreover, ARC is essential for the homeostatic scaling of AMPA receptors, a mechanism that maintains the balance of excitatory signaling in the brain. Beyond its neuronal functions, ARC also contributes to the dynamics of stress fibers and cell migration.
Description
Recombinantly produced in E. coli, the human ARC protein is a single, non-glycosylated polypeptide chain. It consists of 416 amino acids, with a sequence spanning from amino acid position 1 to 396. The protein has a molecular weight of 47.4 kDa. For purification and detection purposes, a 20 amino acid His-tag is fused to the N-terminus of the ARC protein. The protein is purified using proprietary chromatographic techniques to ensure its high quality.
Physical Appearance
The product is a clear and colorless solution that has been sterilized by filtration.
Formulation
The ARC protein solution has a concentration of 0.5mg/ml. It is formulated in a buffer containing 20mM Tris-HCl at pH 8.0, 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (up to four weeks), the protein solution can be stored at 4°C. For extended storage, it is recommended to store the solution in frozen form at -20°C. Adding a carrier protein, such as HSA or BSA, at a concentration of 0.1% is advisable for long-term storage to maintain protein stability. Avoid repeated freezing and thawing of the protein solution.
Purity
The purity of the ARC protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Arg3.1, KIAA0278, Activity-regulated cytoskeleton-associated protein, Activity-regulated gene 3.1 protein homolog, ARC.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MELDHRTSGG LHAYPGPRGG QVAKPNVILQ IGKCRAEMLE HVRRTHRHLL AEVSKQVERE LKGLHRSVGK LESNLDGYVP TSDSQRWKKS IKACLCRCQE TIANLERWVK REMHVWREVF YRLERWADRL ESTGGKYPVG SESARHTVSV GVGGPESYCH EADGYDYTVS PYAITPPPAA GELPGQEPAE AQQYQPWVPG EDGQPSPGVD TQIFEDPREF LSHLEEYLRQ VGGSEEYWLS QIQNHMNGPA KKWWEFKQGS VKNWVEFKKE FLQYSEGTLS REAIQRELDL PQKQGEPLDQ FLWRKRDLYQ TLYVDADEEE IIQYVVGTLQ PKLKRFLRHP LPKTLEQLIQ RGMEVQDDLE QAAEPAGPHL PVEDEAETLT PAPNSESVAS DRTQPE.
Product Science Overview
Introduction
Activity-Regulated Cytoskeleton-Associated Protein (Arc), also known as Arg3.1, is a crucial protein in the field of neurobiology. It is encoded by the ARC gene and is classified as an immediate early gene (IEG) product. Arc plays a significant role in synaptic plasticity, which is essential for learning and memory processes.
Gene and Protein Structure
Function and Mechanism
Arc is involved in the regulation of synaptic strength through several mechanisms:
- AMPA Receptor Endocytosis: Arc facilitates the endocytosis of AMPA receptors (AMPARs) by interacting with the clathrin-adaptor protein 2 (AP-2). This interaction is crucial for the internalization of AMPARs, which modulates synaptic strength .
- Synaptic Plasticity: Arc is essential for activity-dependent synaptic plasticity. It regulates spine density and morphology, which are critical for the structural changes associated with learning and memory .
- Intercellular Communication: Arc can form virus-like capsids that transport RNA between neurons. This retrovirus-like mechanism allows for the transfer of genetic information and plays a role in synaptic plasticity .
Clinical Significance
Research and Applications
Arc is widely studied for its role in neurobiology. It serves as a marker for plastic changes in the brain and is used in various research techniques, such as fluorescence in situ hybridization (FISH). Understanding the mechanisms of Arc can provide insights into therapeutic targets for neurological disorders .
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