APP Antibody

Amyloid Beta A4 Protein, Mouse Anti Human
Cat. No.
BT12364
Source
Synonyms
Amyloid beta A4 protein, Alzheimer disease amyloid protein, ABPP, APPI, APP, PreA4, Cerebral vascular amyloid peptide, CVAP, Protease nexin-II, PN-II, A4, AD1, AAA, PN2, ABETA, CTFgamma.
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Description

Product Specs

Introduction
APP, or Amyloid Precursor Protein, is a transmembrane protein found on the surface of cells. It undergoes cleavage by enzymes called secretases, resulting in the formation of various peptides. Some of these peptides are secreted and can interact with the acetyltransferase complex APBB1/TIP60, leading to the activation of gene transcription. Other peptides derived from APP form the core component of amyloid plaques, which are characteristically observed in the brains of individuals with Alzheimer's disease. Genetic mutations affecting the APP gene have been linked to both familial Alzheimer's disease, inherited in an autosomal dominant manner, and cerebroarterial amyloidosis, also known as cerebral amyloid angiopathy.
Formulation
The antibody is supplied as a 1 mg/ml solution in a buffer composed of Phosphate Buffered Saline (PBS) at a pH of 7.4, with 0.1% Sodium Azide added as a preservative.
Storage Procedures
For short-term storage up to 1 month, keep at 4°C. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C and 1 month when stored at 4°C.
Applications
This APP antibody has been validated for use in ELISA and Western blot applications, demonstrating its specificity and reactivity. As experimental conditions may vary, it is recommended to optimize the antibody concentration for each specific application. For Western blot analysis, a dilution range of 1:1,000 to 1:2,000 is suggested, with an initial starting dilution of 1:1,000.
Synonyms
Amyloid beta A4 protein, Alzheimer disease amyloid protein, ABPP, APPI, APP, PreA4, Cerebral vascular amyloid peptide, CVAP, Protease nexin-II, PN-II, A4, AD1, AAA, PN2, ABETA, CTFgamma.
Purification Method
APP antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PJ4H9AT.
Immunogen
Anti-human APP mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human APP amino acids 18-289 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction

Amyloid Beta A4 Protein, commonly referred to as Amyloid-beta (Aβ), is a peptide that is crucially involved in the pathogenesis of Alzheimer’s disease. The mouse anti-human Amyloid Beta A4 Protein antibody is a monoclonal antibody used extensively in research to study the properties and effects of Amyloid-beta in human samples.

Amyloid Beta A4 Protein

Amyloid-beta is derived from the Amyloid Precursor Protein (APP), a transmembrane protein that is expressed in many tissues, including the brain. APP can be processed through two main pathways: the non-amyloidogenic pathway and the amyloidogenic pathway. The latter involves the sequential cleavage of APP by β-secretase and γ-secretase, resulting in the production of Amyloid-beta peptides of varying lengths, predominantly Aβ40 and Aβ42 .

Role in Alzheimer’s Disease

Amyloid-beta peptides are known to aggregate and form insoluble fibrils that deposit as amyloid plaques in the brains of Alzheimer’s disease patients. These plaques are one of the hallmark pathological features of the disease. The accumulation of Amyloid-beta is believed to disrupt cell-to-cell communication and activate immune responses that lead to inflammation and neuronal cell death .

Mouse Anti-Human Amyloid Beta A4 Protein Antibody

The mouse anti-human Amyloid Beta A4 Protein antibody is a monoclonal antibody that specifically binds to epitopes within the Amyloid-beta peptide. This antibody is used in various applications, including immunoprecipitation (IP), Western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry on frozen sections (IHC-Fr) .

Applications in Research
  1. Immunoprecipitation (IP): This technique allows for the isolation and concentration of Amyloid-beta from complex biological samples, facilitating the study of its properties and interactions.
  2. Western Blotting (WB): Used to detect and quantify Amyloid-beta in samples, providing insights into its expression levels and post-translational modifications.
  3. Immunohistochemistry (IHC): Both IHC-P and IHC-Fr are employed to visualize the distribution and localization of Amyloid-beta in tissue sections, aiding in the understanding of its role in disease pathology.
  4. Immunocytochemistry/Immunofluorescence (ICC/IF): These techniques are used to study the cellular and subcellular localization of Amyloid-beta, offering valuable information on its function and interactions within cells .

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