HEK293 Cells.
ABPP, APPI, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, A4, AD1, beta-amyloid peptide, beta-amyloid precursor protein, testicular tissue protein Li 2, AAA, ABETA, alpha-sApp, CTF gamma, PN2.
Sterile Filtered colorless solution.
> 90% by SDS-PAGE.
APP Human Recombinant is a single, glycosylated, polypeptide chain (18-701 a.a) containing a total of 690 amino acids, having a molecular mass of 78.2 kDa.
APP is fused to a 6 amino acid His-tag at C-terminus and is purified by proprietary chromatographic techniques.
ABPP, APPI, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, A4, AD1, beta-amyloid peptide, beta-amyloid precursor protein, testicular tissue protein Li 2, AAA, ABETA, alpha-sApp, CTF gamma, PN2.
HEK293 Cells.
LEVPTDGNAG LLAEPQIAMF CGRLNMHMNV QNGKWDSDPS GTKTCIDTKE GILQYCQEVY PELQITNVVE ANQPVTIQNW CKRGRKQCKT HPHFVIPYRC LVGEFVSDAL LVPDKCKFLH QERMDVCETH LHWHTVAKET CSEKSTNLHD YGMLLPCGID KFRGVEFVCC PLAEESDNVD SADAEEDDSD VWWGGADTDY ADGSEDKVVE VAEEEEVAEV EEEEADDDED DEDGDEVEEE AEEPYEEATE RTTSIATTTT TTTESVEEVV REVCSEQAET GPCRAMISRW YFDVTEGKCA PFFYGGCGGN RNNFDTEEYC MAVCGSAMSQ SLLKTTQEPL ARDPVKLPTT AASTPDAVDK YLETPGDENE HAHFQKAKER LEAKHRERMS QVMREWEEAE RQAKNLPKAD KKAVIQHFQE KVESLEQEAA NERQQLVETH MARVEAMLND RRRLALENYI TALQAVPPRP RHVFNMLKKY VRAEQKDRQH TLKHFEHVRM VDPKKAAQIR SQVMTHLRVI YERMNQSLSL LYNVPAVAEE IQDEVDELLQ KEQNYSDDVL ANMISEPRIS YGNDALMPSL TETKTTVELL PVNGEFSLDD LQPWHSFGAD SVPANTENEV EPVDARPAAD RGLTTRPGSG LTNIKTEEIS EVKMDAEFRH DSGYEVHHQK LVFFAEDVGS NKGA HHHHHH.
Amyloid beta (A4) precursor protein (APP) is a transmembrane glycoprotein that plays a crucial role in the development of Alzheimer’s disease. The human recombinant version of this protein, produced in Human Embryonic Kidney (HEK) cells, is widely used in research to study its structure, function, and role in disease pathology.
The APP gene is located on chromosome 21 and encodes a protein that can be processed into several isoforms through alternative splicing . The full-length APP protein is approximately 100-140 kDa in size and consists of a large extracellular domain, a single transmembrane domain, and a short intracellular domain .
APP is involved in various physiological processes, including:
APP undergoes proteolytic cleavage by enzymes known as secretases. The cleavage process involves:
The accumulation of Aβ peptides leads to the formation of amyloid plaques, a hallmark of Alzheimer’s disease. These plaques disrupt neuronal function and trigger a cascade of events that result in neurodegeneration . Mutations in the APP gene are associated with familial forms of Alzheimer’s disease and cerebral amyloid angiopathy .
Human recombinant APP produced in HEK cells is used extensively in research to: