ATF Human
Description
Product Specs
Product Science Overview
Apo Transferrin Human is a glycoprotein with a molecular weight ranging from 76 to 81 kDa . It consists of two homologous domains, each capable of binding one molecule of iron. These domains are known as the N-terminal and C-terminal domains, and they exhibit a similar polypeptide folding pattern . The protein is encoded by the TF gene located on chromosome 3q22.1 .
The primary function of Apo Transferrin Human is to transport iron from sites of absorption and storage to various tissues and cells in the body . It binds to iron with high affinity, forming a complex that can be recognized and internalized by transferrin receptors on the cell surface . This process ensures that iron is delivered in a physiologically appropriate manner, maintaining iron homeostasis in the body .
Apo Transferrin Human is an iron-free form of transferrin. It can be supplemented with iron or used to bind free iron present in media . This property makes it an essential component in serum-free cell culture systems, where it serves as a delivery format for iron . The protein is also involved in maintaining the levels of iron in biological fluids and supplying the required iron for hemoglobin synthesis within red blood cells .
Apo Transferrin Human has several applications in research and biotechnology:
- Cell Culture: It is used in serum-free cell culture systems to transport iron into mammalian cells .
- Iron Supplementation: It can be loaded with iron prior to use or added directly to an iron-containing medium .
- Biochemical Research: It is utilized in studies related to iron metabolism, transferrin receptor interactions, and iron homeostasis .
The levels of transferrin in the body can indicate the total iron-binding capacity (TIBC). Iron deficiency, pregnancy, and estrogen administration can increase transferrin levels, while chronic liver disease, renal insufficiency, malnutrition, and protein-losing enteropathies can reduce its synthesis . Understanding the role and function of Apo Transferrin Human is crucial for diagnosing and managing conditions related to iron metabolism.
