TRAIL Human (114-281 a.a.)

TRAIL/APO 2 Ligand (114-281 a.a.) Human Recombinant
Cat. No.
BT29559
Source
Escherichia Coli.
Synonyms
Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.
Appearance
Sterile Filtered colorless liquid.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Soluble TNF-related apoptosis-inducing ligand Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (114-281) and having a molecular mass of 19.6 kDa. The sTRAIL is purified by proprietary chromatographic techniques.

Product Specs

Introduction
TNF-related apoptosis-inducing ligand (TRAIL) is a ligand molecule that initiates apoptosis. Belonging to the tumor necrosis factor family, TRAIL is a type II transmembrane protein. In humans, the gene encoding TRAIL is located on chromosome 3q26. TRAIL binds to death receptors DR4 and DR5, activating a caspase-8-dependent apoptotic pathway. While expressed in various normal tissues, TRAIL exhibits selective apoptosis induction, primarily targeting transformed and tumor cells, with minimal impact on healthy cells.
Description
Soluble TNF-related apoptosis-inducing ligand Human Recombinant, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 169 amino acids (114-281) with a molecular weight of 19.6 kDa. This sTRAIL undergoes purification using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile filtered liquid.
Formulation
Supplied as a 1 mg/ml solution in 20mM Tris-HCl (pH 7.5), 300mM NaCl, 0.1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 95.0% as determined by SDS-PAGE analysis.
Synonyms
Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.
Source
Escherichia Coli.
Amino Acid Sequence
MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNGELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWSKDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG.

Product Science Overview

Structure and Expression

TRAIL is a type II transmembrane protein that can be cleaved to form a soluble protein. The human TRAIL gene is located on chromosome 3q26. The recombinant form of TRAIL, specifically the fragment spanning amino acids 114 to 281, is expressed in Escherichia coli and is often used in research due to its high purity and biological activity .

Mechanism of Action

TRAIL induces apoptosis by binding to its death receptors, DR4 (TRAIL-R1) and DR5 (TRAIL-R2). Upon binding, these receptors undergo trimerization and recruit adaptor proteins such as FADD (Fas-Associated Death Domain), which in turn activate caspase-8. This activation triggers a cascade of downstream caspases, ultimately leading to cell death .

Interestingly, TRAIL can also bind to decoy receptors DcR1 (TRAIL-R3) and DcR2 (TRAIL-R4), which do not induce apoptosis. These decoy receptors can inhibit TRAIL-induced apoptosis by competing with death receptors for TRAIL binding .

Biological Significance

TRAIL is unique among the TNF superfamily members due to its ability to selectively induce apoptosis in cancer cells while sparing normal cells. This selective cytotoxicity makes TRAIL a promising candidate for cancer therapy. Studies have shown that recombinant human TRAIL can induce apoptosis in various cancer cell lines, including those resistant to conventional therapies .

Applications in Research and Medicine

Recombinant human TRAIL (114-281 a.a.) is widely used in research to study apoptosis mechanisms and to develop potential cancer therapies. Its high purity and biological activity make it suitable for various applications, including:

  • SDS-PAGE: For analyzing protein purity and molecular weight.
  • Functional Studies (FuncS): To assess the biological activity of TRAIL in inducing apoptosis in cancer cell lines .

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