APIP Human

APAF1 interacting protein Human Recombinant
Cat. No.
BT24641
Source
E.coli.
Synonyms
APAF1 interacting protein, CGI-29, APIP2, Mmrp19, APAF1-interacting protein, MTRu-1-P dehydratase, probable methylthioribulose-1-phosphate dehydratase, CGI29, EC 4.2.1.109, dJ179L10.2, MMRP19.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

APIP Human Recombinant produced E. coli is a single polypeptide chain containing 266 amino acids (1-242) and having a molecular mass of 29.7kDa.
APIP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
APIP, an enzyme, plays a crucial role in the methionine salvage pathway by catalyzing the conversion of methylthioribulose-1-phosphate (MTRu-1-P) to 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Beyond its enzymatic function, APIP exhibits anti-apoptotic properties, protecting cells from programmed death by inhibiting both cytochrome c-dependent and APAF1-mediated pathways. This protective effect has been linked to reduced muscle damage following ischemia.
Description
This product consists of the recombinant human APIP protein, expressed in E. coli and purified to a high degree. The protein encompasses amino acids 1 to 242 of the APIP sequence, with an additional 24-amino acid His-tag fused at the N-terminus. The molecular weight of the tagged protein is 29.7kDa. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The APIP protein is provided at a concentration of 0.5mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein like HSA or BSA to a final concentration of 0.1% is advisable. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the APIP protein in this product is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
APAF1 interacting protein, CGI-29, APIP2, Mmrp19, APAF1-interacting protein, MTRu-1-P dehydratase, probable methylthioribulose-1-phosphate dehydratase, CGI29, EC 4.2.1.109, dJ179L10.2, MMRP19.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSGCDA REGDCCSRRC GAQDKEHPRY LIPELCKQFY HLGWVTGTGG GISLKHGDEI YIAPSGVQKE RIQPEDMFVC DINEKDISGP SPSKKLKKSQ CTPLFMNAYT MRGAGAVIHT HSKAAVMATL LFPGREFKIT HQEMIKGIKK CTSGGYYRYD DMLVVPIIEN TPEEKDLKDR MAHAMNEYPD SCAVLVRRHG VYVWGETWEK AKTMCECYDY LFDIAVSMKK VGLDPSQLPV GENGIV

Product Science Overview

Structure and Function

APAF1 contains several important domains:

  • WD-40 repeats: These are involved in protein-protein interactions.
  • Caspase Recruitment Domain (CARD): This domain is essential for the interaction with procaspase-9.
  • ATPase domain (NB-ARC): This domain is involved in the oligomerization of APAF1.

Upon binding to cytochrome c and dATP, APAF1 undergoes a conformational change that allows it to oligomerize and form the apoptosome, a large quaternary protein structure. The apoptosome then recruits and activates procaspase-9, which in turn activates the executioner caspases, leading to apoptosis .

APAF1 Interacting Protein

The APAF1 interacting protein (Human Recombinant) is a synthetic version of the naturally occurring protein that interacts with APAF1. This interaction is crucial for the formation and function of the apoptosome. The recombinant version is produced using recombinant DNA technology, which involves inserting the gene encoding the APAF1 interacting protein into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities.

Biological Significance

The interaction between APAF1 and its interacting proteins is vital for the regulation of apoptosis. Dysregulation of apoptosis can lead to various diseases, including cancer, autoimmune disorders, and neurodegenerative diseases. Therefore, understanding the interactions of APAF1 is crucial for developing therapeutic strategies for these conditions .

Research and Applications

Recombinant APAF1 interacting protein is widely used in research to study the mechanisms of apoptosis. It is also used in drug discovery and development, as targeting the apoptotic pathway is a promising strategy for treating diseases characterized by either excessive or insufficient apoptosis.

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THE BIOTEK
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