APEX1 Human
APEX Nuclease-1 Human Recombinant
Cat. No.
BT13584
Source
Escherichia Coli.
Synonyms
APEX nuclease (multifunctional DNA repair enzyme) 1, APE, REF1, HAP1, APX, APEN, APEX, Apurinic-apyrimidinic endonuclease 1, Protein REF-1, AP endonuclease 1, APEX nuclease (multifunctional DNA repair enzyme), APE-1, deoxyribonuclease (apurinic or apyrimidinic), apurinic/apyrimidinic exonuclease, AP endonuclease class I, multifunctional DNA repair enzyme, redox factor 1, EC 4.2.99.18.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
APEX1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 332 amino acids (1-318a.a.) and having a molecular mass of 36.9kDa.
APEX1 protein is fused to a 14 amino acid T7-tag at N-terminus and is purified by standard chromatography.
APEX1 protein is fused to a 14 amino acid T7-tag at N-terminus and is purified by standard chromatography.
Product Specs
Introduction
APEX1 is an enzyme responsible for initiating the base excision repair pathway by cleaving DNA at specific sites. It also enhances the DNA binding capability of various transcription factors involved in cancer development and progression. APEX1 plays a crucial role in the cellular response to oxidative stress, safeguarding cells against the harmful effects of oxidizing agents.
Description
Recombinant human APEX1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 332 amino acids (residues 1-318) and possessing a molecular weight of 36.9 kDa. This protein is engineered with a 14-amino acid T7-tag at the N-terminus and purified using standard chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
Human APEX1 solution (1 mg/ml) buffered in 20 mM Tris-HCl at pH 8, 2 mM DTT, 0.2 M NaCl, and 10% glycerol.
Purity
Purity exceeds 90% as assessed by SDS-PAGE.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Avoid repeated freeze-thaw cycles to maintain product integrity.
Synonyms
APEX nuclease (multifunctional DNA repair enzyme) 1, APE, REF1, HAP1, APX, APEN, APEX, Apurinic-apyrimidinic endonuclease 1, Protein REF-1, AP endonuclease 1, APEX nuclease (multifunctional DNA repair enzyme), APE-1, deoxyribonuclease (apurinic or apyrimidinic), apurinic/apyrimidinic exonuclease, AP endonuclease class I, multifunctional DNA repair enzyme, redox factor 1, EC 4.2.99.18.
Source
Escherichia Coli.
Amino Acid Sequence
MASMTGGQQM GRGSMPKRGK KGAVAEDGDE LRTEPEAKKS KTAAKKNDKE AAGEGPALYE DPPDQKTSPS GKPATLKICS WNVDGLRAWI KKKGLDWVKE EAPDILCLQE TKCSENKLPA ELQELPGLSH QYWSAPSDKE GYSGVGLLSR QCPLKVSYGI GEEEHDQEGR VIVAEFDSFV LVTAYVPNAG RGLVRLEYRQ RWDEAFRKFL KGLASRKPLV LCGDLNVAHE EIDLRNPKGN KKNAGFTPQE RQGFGELLQA VPLADSFRHL YPNTPYAYTF WTYMMNARSK NVGWRLDYFL LSHSLLPALC DSKIRSKALG SDHCPITLYL AL.
Product Science Overview
Structure and Function
APEX1 is a multifunctional enzyme with several activities:
- AP Endonuclease Activity: It cleaves the phosphodiester backbone at AP sites, which are locations in DNA where the base has been removed, leaving a deoxyribose sugar without a base.
- 3’-5’ Exonuclease Activity: It removes nucleotides from the ends of DNA strands.
- DNA 3’ Repair Diesterase Activity: It processes the 3’ ends of DNA to prepare them for further repair.
- DNA 3’-Phosphatase Activity: It removes phosphate groups from the 3’ ends of DNA .
These activities are essential for the base excision repair (BER) pathway, which is responsible for correcting small base lesions resulting from oxidation and alkylation damage.
Expression and Localization
APEX1 is ubiquitously expressed in human cells, with varying levels across different tissues. High expression levels are observed in the testis, thymus, spleen, kidney, and brain, while lower levels are found in the liver . This distribution suggests that APEX1 plays a vital role in protecting cells from DNA damage in tissues with high metabolic activity.
Recombinant APEX1
Applications
Recombinant APEX1 is used in various research applications, including:
- DNA Repair Studies: Understanding the mechanisms of DNA repair and the role of APEX1 in maintaining genomic stability.
- Drug Development: Screening for potential inhibitors or activators of APEX1 that could be used in cancer therapy.
- Biochemical Assays: Investigating the enzyme’s activities and interactions with other proteins involved in DNA repair .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
