ANXA5 Mouse
Description
ANXA5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Annexin A5, also known as ANXA5 or annexin V, is a cellular protein belonging to the annexin family. This family comprises over 160 proteins characterized by their ability to bind to negatively charged phospholipid surfaces in a calcium-dependent manner . Annexin A5 is widely studied for its role in various biological processes, including apoptosis, blood coagulation, and cellular signaling.
Annexin A5 was first isolated from human placenta in the late 1970s . The protein has a molecular weight of approximately 35 kDa and consists of four annexin repeats, which are responsible for its calcium-dependent phospholipid-binding properties . The recombinant form of Annexin A5, derived from mouse, is commonly used in research to study its functions and applications.
- Apoptosis Detection: Annexin A5 is widely used as a probe to detect apoptotic cells. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. Annexin A5 binds to PS with high affinity, making it a valuable tool for identifying apoptotic cells in various assays .
- Blood Coagulation: Annexin A5 plays a role in inhibiting blood coagulation by competing with prothrombin for binding sites on phosphatidylserine. This property helps regulate the coagulation cascade and prevent excessive clot formation .
- Cellular Signaling: Annexin A5 is involved in various cellular signaling pathways. It interacts with proteins such as kinase insert domain receptor and integrin beta 5, influencing processes like cell adhesion, migration, and proliferation .
Annexin A5 has significant potential in clinical applications, particularly in the fields of cardiology and oncology. Radiolabeled Annexin A5 can be used as a diagnostic tool to visualize cell death in conditions such as myocardial infarction, atherosclerosis, and cancer . Additionally, it may serve as a therapeutic agent for monitoring and targeting cell death-inducing therapies.
In the laboratory, Annexin A5 is commonly used in flow cytometry and fluorescence microscopy to detect apoptotic cells. The Annexin A5 affinity assay typically involves conjugating Annexin A5 with a fluorescent or enzymatic label, allowing for the distinction between viable, apoptotic, and necrotic cells . This assay is widely used in research to study cell death mechanisms and evaluate the efficacy of therapeutic agents.
