SSU72 PAT45E2AT Antibody
SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae), HSPC182, CTD phosphatase SSU72, Ssu72 RNA polymerase II CTD phosphatase homolog (yeast), PNAS-120, RNA polymerase II subunit A C-terminal domain phosphatase SSU72, EC 3.1.3.16.
Description
Product Specs
SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae), HSPC182, CTD phosphatase SSU72, Ssu72 RNA polymerase II CTD phosphatase homolog (yeast), PNAS-120, RNA polymerase II subunit A C-terminal domain phosphatase SSU72, EC 3.1.3.16.
SSU72 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT45E2AT.
Anti-human SSU72 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human SSU72 amino acids 1-194 purified from E. coli.
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
SSU72 RNA Polymerase II CTD Phosphatase is a crucial enzyme involved in the regulation of gene expression. The enzyme is encoded by the SSU72 gene, which is conserved across eukaryotic species . This article delves into the structure, function, and significance of SSU72, as well as the specific monoclonal antibody clone PAT45E2AT, which is used for research purposes.
SSU72 is a protein phosphatase that specifically targets the C-terminal domain (CTD) of RNA Polymerase II . The CTD consists of heptapeptide repeats that undergo phosphorylation and dephosphorylation cycles during the transcription process. SSU72 dephosphorylates the Ser5 residue of these repeats, playing a pivotal role in the transition from transcription initiation to elongation .
The enzyme is also involved in mRNA processing and termination, interacting with various transcription factors and components of the polyadenylation machinery . SSU72’s activity is essential for the proper regulation of gene expression and RNA processing.
SSU72’s role in dephosphorylating RNA Polymerase II CTD is critical for the regulation of transcription cycles . By modulating the phosphorylation status of the CTD, SSU72 ensures the proper progression of transcription and the timely processing of pre-mRNA. This regulation is vital for maintaining cellular homeostasis and responding to various cellular signals .
Clone PAT45E2AT is a mouse monoclonal antibody specifically designed to target human SSU72 . Monoclonal antibodies are produced by identical immune cells that are clones of a unique parent cell, ensuring specificity and consistency in their binding properties.
Applications
The PAT45E2AT clone is widely used in various research applications, including:
Mouse anti-human antibodies, like clone PAT45E2AT, are commonly used in research due to their high specificity and affinity for human proteins . These antibodies are generated by immunizing mice with human antigens, leading to the production of antibodies that can be harvested and purified for research use.
However, the use of mouse antibodies in human applications can sometimes lead to the Human Anti-Mouse Antibody (HAMA) response, where the human immune system recognizes the mouse antibodies as foreign and mounts an immune response . This can affect the efficacy and safety of therapeutic applications, but it is less of a concern in basic research settings.
