ANP32A Human
Acidic Nuclear Phosphoprotein 32 Family Member A Human Recombinant
Cat. No.
BT24347
Source
Escherichia Coli.
Synonyms
I1PP2A, LANP, MAPM, PHAP1, Leucine-rich acidic nuclear protein, PP32, Mapmodulin.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ANP32A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 269 amino acids (1-249a.a.) and having a molecular mass of 30.7kDa.
ANP32A is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
ANP32A is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
ANP32A frequently collaborates with MAP1B, TAF1A, and Protein SET. It plays a role in various cellular functions, including proliferation, differentiation, and both caspase-dependent and caspase-independent apoptosis. Mapmodulin participates in E4F1-mediated transcriptional repression.
Description
Recombinant human ANP32A, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 269 amino acids (residues 1-249) and possessing a molecular weight of 30.7 kDa. It includes a 20 amino acid His Tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ANP32A solution is provided at a concentration of 0.5 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 100 mM NaCl, 0.1 mM PMSF, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Minimize repeated freeze-thaw cycles.
Purity
Purity exceeds 95% as determined by SDS-PAGE analysis.
Synonyms
I1PP2A, LANP, MAPM, PHAP1, Leucine-rich acidic nuclear protein, PP32, Mapmodulin.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MEMGRRIHLE LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI NVGLTSIANL PKLNKLKKLE LSDNRVSGGL EVLAEKCPNL THLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDE EEDEDEEEYD EDAQVVEDEE DEDEEEEGEE EDVSGEEEED EEGYNDGEVD DEEDEEELGE EERGQKRKRE PEDEGEDDD.
Product Science Overview
Gene and Protein Structure
Functions and Biological Roles
ANP32A is involved in several critical cellular processes, including:
- Tumor Suppression: ANP32A has been shown to promote apoptosis by favoring the activation of caspase-9 and allowing apoptosome formation .
- Regulation of Transcription: It plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex .
- Nucleocytoplasmic Transport: ANP32A is involved in the transport of molecules between the nucleus and the cytoplasm .
- RNA Binding: It has RNA binding activity, which is crucial for its role in regulating mRNA stability and processing .
Clinical Significance
Research and Applications
The recombinant form of ANP32A is used in research to study its functions and interactions with other proteins. It is also a target for developing therapeutic interventions for diseases associated with its dysregulation.
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