ANGPTL4 Human, HEK
Angiopoietin-like Protein 4 Human Recombinant, HEK
Cat. No.
BT10474
Source
HEK293.
Synonyms
ANGPTL4, NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2, Fasting- Induced Adipose Factor, Hepatic Fibrinogen/Angiopoietin-Related Protein, PPARG Angiopoietin-Related Protein.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
The ANGPTL4 Human Recombinant is manufactured with C-terminal fusion of 11 amino acid FLAG Tag.
The ANGPTL4 Flag -Tagged Fusion Protein is a 44.2kDa protein containing 392 amino acid residues of the Angiopoietin-like Protein 4 and 11 additional amino acid residues - Flag Tag (underlined).
The ANGPTL4 Flag -Tagged Fusion Protein is a 44.2kDa protein containing 392 amino acid residues of the Angiopoietin-like Protein 4 and 11 additional amino acid residues - Flag Tag (underlined).
Product Specs
Introduction
Fasting-induced adipose factor (FIAF), also known as ANGPTL4, PGAR, or HFARP, is an adipocytokine that increases with fasting, peroxisome proliferator-activated receptor agonists, and hypoxia. In humans and mice, FIAF exists in both native and truncated forms within blood plasma. While truncated FIAF is primarily observed in mouse 3T3-L1 adipocyte differentiation, human white adipose tissue and SGBS adipocytes only produce the native form. Notably, the truncated form is produced in the human liver. Studies suggest that truncated FIAF (FIAF-S2) is the predominant form in human blood plasma, with levels increasing upon fenofibrate treatment. Despite significant individual variations, both FIAF forms exhibit no correlation with body mass index and remain unaffected by prolonged semi-starvation.
Description
The ANGPTL4 Human Recombinant protein, with a C-terminal 11 amino acid FLAG Tag, has a molecular weight of 44.2kDa. It consists of 392 amino acids from the Angiopoietin-like Protein 4 and an additional 11 amino acids forming the Flag Tag (underlined).
Physical Appearance
White, lyophilized powder that has been filtered.
Formulation
The product is filtered and lyophilized from a solution of 20mM Tris buffer and 50mM NaCl at pH 7.5, with a concentration of 0.5mg/ml.
Solubility
To achieve a working concentration of 0.5mg/ml, add pyrogen-free water to the lyophilized pellet and ensure complete dissolution. Note: This product is not sterile. Prior to cell culture use, filter the solution through an appropriate sterile filter.
Stability
Store the lyophilized Angiopoietin-like Protein 4 Human recombinant at -20°C. After reconstitution, aliquot the product to minimize freeze-thaw cycles. The reconstituted protein remains stable at 4°C for a limited period, showing no changes for up to two weeks.
Purity
The purity of the protein is determined to be greater than 90% using SDS-PAGE analysis.
Applications
This product is suitable for Western blotting applications.
Synonyms
ANGPTL4, NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2, Fasting- Induced Adipose Factor, Hepatic Fibrinogen/Angiopoietin-Related Protein, PPARG Angiopoietin-Related Protein.
Source
HEK293.
Amino Acid Sequence
GPVQSKSPRF ASWDEMNVLA HGLLQLGQGL REHAERTRSQ LSALERRLSA CGSACQGTEG STDLPLAPES RVDPEVLHSL QTQLKAQNSR IQQLFHKVAQ QQRHLEKQHL RIQHLQSQFG LLDHKHLDHE VAKPARRKRL PEMAQPVDPA HNVSRLHRLP RDCQELFQVG ERQSGLFEIQ PQGSPPFLVN CKMTSDGGWT VIQRRHDGSV DFNRPWEAYK AGFGDPHGEF WLGLEKVHSI TGDRNSRLAV QLRDWDGNAE LLQFSVHLGG EDTAYSLQLT APVAGQLGAT TVPPSGLSVP FSTWDQDHDL RRDKNCAKSL SGGWWFGTCS HSNLNGQYFR SIPQQRQKLK KGIFWKTWRG RYYPLQATTM LIQPMAAEAA SAAADYKDDDDK.
Product Science Overview
Structure and Expression
ANGPTL4 is a secreted protein that consists of two main domains:
- N-terminal coiled-coil domain
- C-terminal fibrinogen-like domain
These domains can be proteolytically separated in vivo . The protein is glycosylated and has a molecular weight of approximately 55 kDa . In humans, ANGPTL4 is expressed in various tissues, including the liver, adipose tissue, and muscle .
Functions
ANGPTL4 is involved in several key biological processes:
- Lipid Metabolism: ANGPTL4 inhibits the enzyme lipoprotein lipase (LPL), which plays a crucial role in the hydrolysis of triglycerides in lipoproteins. By inhibiting LPL, ANGPTL4 regulates triglyceride clearance from the blood serum .
- Angiogenesis: ANGPTL4 modulates angiogenesis, the process of forming new blood vessels, by inhibiting endothelial cell proliferation, migration, and tubule formation .
- Glucose Homeostasis and Insulin Sensitivity: ANGPTL4 may also play a role in regulating glucose levels and insulin sensitivity .
- Cancer: ANGPTL4 is implicated in various cancers, where it modulates vascular permeability, cancer cell motility, and invasiveness .
Recombinant ANGPTL4 (Human, HEK)
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