ANAPC13 Human

Anaphase Promoting Complex Subunit 13 Human Recombinant

Cat. No.

BT24172

Source

Escherichia Coli.

Synonyms

Anaphase-promoting complex subunit 13, APC13, Cylosome subunit 13, ANAPC13, SWM1.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

ANAPC13 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 89 amino acids (1-74) and having a molecular mass of 10kDa (Molecular weight on SDS-PAGE will appear higher).
ANAPC13 protein is fused to a 15 amino acid T7-tag at N-terminus and is purified by standard chromatography.

Product Specs

Introduction

Anaphase-promoting complex subunit 13 (ANAPC13) is a protein that is part of a larger complex called the anaphase promoting complex. This complex is responsible for controlling the cell cycle, which is the series of events that lead to cell growth and division. It does this by regulating the breakdown of specific proteins that control the cell cycle, such as B-type cyclins. ANAPC13 is found in many different organisms and is crucial for the anaphase promoting complex to function correctly.

Description

This product contains the human ANAPC13 protein, which has been produced in E. coli bacteria. It is a single chain of 89 amino acids (building blocks of proteins) and has a molecular weight of 10kDa. The protein is not glycosylated, meaning it doesn't have sugars attached to it. To aid in purification and detection, a 15 amino acid T7-tag is attached to the beginning of the protein. The protein is purified using standard chromatography techniques.

Physical Appearance

Clear solution without any particles, sterilized by filtration.

Formulation

This solution contains 1mg/ml of ANAPC13 protein dissolved in a buffer containing 20mM Tris-HCl (pH 8.0), 20% glycerol, 1mM DTT, and 0.1M NaCl.

Stability

For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.

Purity

More than 90% pure as measured by SDS-PAGE, a technique used to analyze protein purity.

Synonyms

Anaphase-promoting complex subunit 13, APC13, Cylosome subunit 13, ANAPC13, SWM1.

Source

Escherichia Coli.

Amino Acid Sequence

MASMTGGQQM GRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYL HENVPPIGN.

Product Science Overview

Introduction

The Anaphase Promoting Complex (APC), also known as the cyclosome, is a crucial E3 ubiquitin ligase that regulates the cell cycle by marking specific proteins for degradation by the 26S proteasome. One of its essential subunits is the Anaphase Promoting Complex Subunit 13 (ANAPC13), which plays a significant role in maintaining the integrity and functionality of the APC/C complex .

Structure and Function

ANAPC13 is a protein-coding gene that encodes a component of the APC/C complex. The APC/C complex is composed of 11-13 subunit proteins, including a cullin (Apc2) and RING (Apc11) subunit, which are essential for its ubiquitin ligase activity. ANAPC13 is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the APC/C complex.

The primary function of the APC/C complex is to control cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. This regulation is achieved through the ubiquitination and subsequent degradation of target proteins, primarily mediating the formation of ‘Lys-11’-linked polyubiquitin chains .

Biological Significance

ANAPC13 is crucial for the proper functioning of the APC/C complex, which in turn is vital for cell cycle regulation. The APC/C complex controls progression through mitosis and the G1 phase of the cell cycle by triggering the degradation of key cell cycle regulators. This ensures that cells divide correctly and maintain genomic stability.

Clinical Relevance

Mutations or dysregulation of ANAPC13 and other components of the APC/C complex can lead to various diseases. For instance, diseases associated with ANAPC13 include Neurofibromatosis, Familial Spinal, and Middle Ear Adenoma. Understanding the role of ANAPC13 in these diseases can provide insights into potential therapeutic targets for treating cell cycle-related disorders.

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ANAPC13 Human

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