AMPD2 Human

AMPD2 Human Recombinant
Cat. No.
BT4803
Source
Escherichia Coli.
Synonyms

(Isoform L), EC 3.5.4.6, SPG63, AMP Deaminase Isoform L, AMP Deaminase 2, AMPD Isoform L, AMPD, PCH9, AMP deaminase 2.

Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by Analysis by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

AMPD2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 667 amino acids (236-879 a.a) and having a molecular mass of 77.0kDa.
AMPD2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
AMPD2 plays a crucial role in purine metabolism by facilitating the conversion of AMP to IMP. This enzyme, functioning as a homotetramer, represents one of the three identified AMP deaminases found in mammals. Notably, multiple transcript variants, responsible for encoding different isoforms of AMPD2, have been discovered.
Description
Recombinant human AMPD2, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 667 amino acids (specifically, residues 236-879). It possesses a molecular mass of 77.0 kDa. The protein is engineered with a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The AMPD2 protein is provided in a solution at a concentration of 0.25 mg/ml. The solution is buffered with phosphate-buffered saline (pH 7.4) and contains 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, adding a carrier protein (either 0.1% HSA or BSA) is advisable. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the AMPD2 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms

(Isoform L), EC 3.5.4.6, SPG63, AMP Deaminase Isoform L, AMP Deaminase 2, AMPD Isoform L, AMPD, PCH9, AMP deaminase 2.

Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSDLLDAAK SVVRALFIRE KYMALSLQSF CPTTRRYLQQ LAEKPLETRT YEQGPDTPVS ADAPVHPPAL EQHPYEHCEP STMPGDLGLG LRMVRGVVHV YTRREPDEHC SEVELPYPDL QEFVADVNVL MALIINGPIK SFCYRRLQYL SSKFQMHVLL NEMKELAAQK KVPHRDFYNI RKVDTHIHAS SCMNQKHLLR FIKRAMKRHL EEIVHVEQGR EQTLREVFES MNLTAYDLSV DTLDVHADRN TFHRFDKFNA KYNPIGESVL REIFIKTDNR VSGKYFAHII KEVMSDLEES KYQNAELRLS IYGRSRDEWD KLARWAVMHR VHSPNVRWLV QVPRLFDVYR TKGQLANFQE MLENIFLPLF EATVHPASHP ELHLFLEHVD GFDSVDDESK PENHVFNLES PLPEAWVEED NPPYAYYLYY TFANMAMLNH LRRQRGFHTF VLRPHCGEAG PIHHLVSAFM LAENISHGLL LRKAPVLQYL YYLAQIGIAM SPLSNNSLFL SYHRNPLPEY LSRGLMVSLS TDDPLQFHFT KEPLMEEYSI ATQVWKLSSC DMCELARNSV LMSGFSHKVK SHWLGPNYTK EGPEGNDIRR TNVPDIRVGY RYETLCQELA LITQAVQSEM LETIPEEAGI TMSPGPQ.

Product Science Overview

Gene and Protein Structure

The AMPD2 gene is located on chromosome 1 in humans and encodes the AMP deaminase 2 protein. The protein consists of 825 amino acids and functions as a homotetramer, meaning it forms a complex of four identical subunits . The enzyme requires zinc ions as cofactors for its catalytic activity .

Biological Function

AMPD2 is one of three AMP deaminases found in mammals, each with distinct tissue distributions and physiological roles. AMPD2 is predominantly expressed in the brain and plays a significant role in maintaining the balance of adenine nucleotides, which are critical for various cellular processes .

Recombinant AMPD2

Recombinant AMPD2 refers to the AMPD2 protein that has been produced using recombinant DNA technology. This involves inserting the AMPD2 gene into an expression system, such as Escherichia coli, to produce the protein in large quantities . The recombinant protein is often tagged with a His-tag at the N-terminus to facilitate purification and is typically used in research to study the enzyme’s structure, function, and potential therapeutic applications .

Clinical Significance

Mutations in the AMPD2 gene have been associated with several neurological disorders, including pontocerebellar hypoplasia type 9 (PCH9) and spastic paraplegia type 63 (SPG63). These conditions are characterized by structural defects in the brain, delayed psychomotor development, and progressive weakness and spasticity of the lower limbs .

Research and Applications

Recombinant AMPD2 is widely used in biochemical and cellular assays to understand its role in purine metabolism and its potential as a therapeutic target. The enzyme’s activity can be measured using various techniques, including SDS-PAGE and ELISA .

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