AMN Human

Amnion Associated Transmembrane Protein Human Recombinant
Cat. No.
BT23994
Source
Escherichia Coli.
Synonyms
Protein amnionless, AMN, PRO1028, amnionless.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

AMN Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 361 amino acids (20-357 a.a) and having a molecular mass of 38.2kDa.
AMN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Amnion Associated Transmembrane Protein (AMN) is a type I transmembrane protein responsible for the efficient absorption of vitamin B12. It is believed to play a role in modulating bone morphogenetic protein (BMP) receptor function, potentially acting as an accessory or coreceptor that influences BMP binding. AMN forms the cubam receptor when complexed with cubilin.
Description
Recombinant human AMN, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 361 amino acids (specifically, amino acids 20-357). It has a molecular mass of 38.2 kDa. The AMN protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution.
Formulation
The AMN protein solution has a concentration of 0.5 mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Protein amnionless, AMN, PRO1028, amnionless.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSVSKLWVP NTDFDVAANW SQNRTPCAGG AVEFPADKMV SVLVQEGHAV SDMLLPLDGE LVLASGAGFG VSDVGSHLDC GAGEPAVFRD SDRFSWHDPH LWRSGDEAPG LFFVDAERVP CRHDDVFFPP SASFRVGLGP GASPVRVRSI SALGRTFTRD EDLAVFLASR AGRLRFHGPG ALSVGPEDCA DPSGCVCGNA EAQPWICAAL LQPLGGRCPQ AACHSALRPQ GQCCDLCGAV VLLTHGPAFD LERYRARILD TFLGLPQYHG LQVAVSKVPR SSRLREADTE IQVVLVENGP ETGGAGRLAR ALLADVAENG EALGVLEATM RESGAHVWGS S.

Product Science Overview

Introduction

Amnion Associated Transmembrane Protein (AMN) is a type I transmembrane protein encoded by the AMN gene. This protein plays a crucial role in various biological processes, including the modulation of bone morphogenetic protein (BMP) receptor function. It acts as an accessory or coreceptor, facilitating or hindering BMP binding .

Gene and Protein Structure

The AMN gene is located on chromosome 14 and is responsible for coding the AMN protein. The protein has a molecular weight of approximately 47.7 kDa and is expressed in various tissues, including the extraembryonic visceral endoderm layer during gastrulation . The AMN protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila .

Function and Mechanism

AMN is a membrane-bound component of the endocytic receptor formed by AMN and CUBN (Cubilin). This complex is essential for the efficient absorption of vitamin B12 and normal CUBN-mediated protein transport in the kidney . The AMN-CUBN complex is also required for normal CUBN glycosylation and trafficking to the cell surface .

Clinical Significance

Mutations in the AMN gene are associated with several diseases, including Imerslund-Grasbeck Syndrome 2 and Megaloblastic Anemia . These conditions are characterized by impaired vitamin B12 absorption, leading to various hematological and neurological symptoms.

Research and Applications

Human recombinant AMN protein is used extensively in research to study its role in BMP receptor function and its involvement in various diseases. The protein is typically expressed in HEK293 cells and is available in various formats for research purposes . It is important to note that the recombinant AMN protein is for research use only and is not approved for clinical diagnosis or therapeutic use .

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