AMIGO2 Human

Adhesion Molecule with Ig-Like Domain 2 Human Recombinant
Cat. No.
BT23793
Source
Sf9, Insect cells.
Synonyms
Adhesion Molecule with Ig-Like Domain 2, Differentially Expressed in Gastric, Adenocarcinomas, AMIGO-2, ALI1, DEGA, Differentially Expressed in Gastric, Adenocarcinoma, Amphoterin-Induced Gene and Open Reading Frame 2, Transmembrane Protein AMIGO2, Amphoterin Induced Gene, Alivin 1, Alivin-1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

AMIGO2 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 367 amino acids (40-398a.a.) and having a molecular mass of 41.9kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
AMIGO2 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Adhesion Molecule with Ig-Like Domain 2, also known as AMIGO2, belongs to the leucine-rich repeat protein family. This molecule facilitates both homophilic interactions (binding to itself) and heterophilic interactions (binding to AMIGO1 or AMIGO3), contributing to cell-cell adhesion. Additionally, AMIGO2 plays a role in signal transduction pathways through its intracellular domain. Importantly, AMIGO2 has been implicated in the development of certain gastric adenocarcinomas.
Description
Recombinant AMIGO2 protein, expressed in Sf9 insect cells, is a single, glycosylated polypeptide chain with a molecular weight of 41.9 kDa. This protein encompasses amino acids 40 to 398 and includes an 8-amino acid His tag at the C-terminus. Purification is achieved using proprietary chromatographic techniques. It's important to note that the observed molecular size on SDS-PAGE may vary from 40 to 57 kDa due to glycosylation.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The AMIGO2 protein solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the AMIGO2 protein should be kept at 4°C. For longer storage, it is recommended to freeze the protein at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advisable. Avoid repeatedly freezing and thawing the protein solution.
Purity
The purity of the AMIGO2 protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Adhesion Molecule with Ig-Like Domain 2, Differentially Expressed in Gastric, Adenocarcinomas, AMIGO-2, ALI1, DEGA, Differentially Expressed in Gastric, Adenocarcinoma, Amphoterin-Induced Gene and Open Reading Frame 2, Transmembrane Protein AMIGO2, Amphoterin Induced Gene, Alivin 1, Alivin-1.
Source
Sf9, Insect cells.
Amino Acid Sequence
VCPTACICAT DIVSCTNKNL SKVPGNLFRL IKRLDLSYNR IGLLDSEWIP VSFAKLNTLI
LRHNNITSIS TGSFSTTPNL KCLDLSSNKL KTVKNAVFQE LKVLEVLLLY NNHISYLDPS AFGGLSQLQK LYLSGNFLTQ FPMDLYVGRF KLAELMFLDV SYNRIPSMPM HHINLVPGKQ LRGIYLHGNP FVCDCSLYSL LVFWYRRHFS SVMDFKNDYT CRLWSDSRHS RQVLLLQDSF MNCSDSIING SFRALGFIHE AQVGERLMVH CDSKTGNANT DFIWVGPDNR LLEPDKEMEN FYVFHNGSLV IESPRFEDAG VYSCIAMNKQ RLLNETVDVT INVSNFTVSR SHAHEAFNTL EHHHHHH.

Product Science Overview

Structure and Function

AMIGO2 is a transmembrane protein that contains several distinct domains:

  • N-terminal signal sequence
  • Cysteine-rich domains
  • Leucine-rich repeats (LRRs)
  • Immunoglobulin C2-like loop
  • Transmembrane region
  • C-terminal intracellular domain

These structural features enable AMIGO2 to mediate both homophilic (self-binding) and heterophilic (binding with different molecules) cell-cell interactions. It can interact with other members of the AMIGO family, such as AMIGO1 and AMIGO3 .

Biological Role

AMIGO2 plays a crucial role in the survival and function of neurons. It is required for the depolarization-dependent survival of cultured cerebellar granule neurons. Additionally, AMIGO2 is involved in the positive regulation of synapse assembly and may contribute to signal transduction through its intracellular domain .

Clinical Significance

AMIGO2 has been identified as a biomarker for gastric adenocarcinoma, a type of stomach cancer. It is differentially expressed in gastric adenocarcinomas and may be required for the tumorigenesis of a subset of these cancers . Furthermore, AMIGO2 is associated with Parkinson Disease 7, Autosomal Recessive Early-Onset .

Research and Applications

Recombinant human AMIGO2 is used in various research applications to study its function and role in diseases. It is particularly valuable in understanding the mechanisms of cell adhesion, neuronal survival, and cancer progression .

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