AMELX Human
Description
AMELX is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Amelogenins are extracellular matrix proteins that, along with other proteins like ameloblastins, enamelins, and tuftelins, direct the mineralization of enamel to form a highly organized matrix of rods and interrod crystal proteins . During the development of human enamel, amelogenins constitute about 90% of the total protein content . They are believed to regulate the initiation and growth of hydroxyapatite crystals, which are essential for the mineralization process .
The AMELX gene is located on the X chromosome at Xp22.1–Xp22.3 . Mutations in this gene can lead to a rare genetic condition known as Amelogenesis Imperfecta 1E (AI1E), characterized by abnormal tooth enamel development . The gene’s location on the X chromosome means that males (with one X chromosome) and females (with two X chromosomes) can exhibit different patterns of inheritance and expression of amelogenin .
Recombinant amelogenin, specifically the X-linked human isoform, is produced using Escherichia coli (E. coli) expression systems . This recombinant protein is used in various research and therapeutic applications. It is supplied as a lyophilized powder, prepared from a filtered solution of amelogenin in 2% acetic acid without any additives . The biological activity of recombinant amelogenin is measured by its ability to promote the attachment of Saos-2 cells to a coated surface .
Amelogenin has several potential therapeutic applications, including:
- Treatment of hard-to-heal wounds: Amelogenin acts as a growth factor for mesenchymal stem cells, promoting tissue regeneration .
- Remineralization of caries lesions: It helps in the repair and remineralization of tooth enamel affected by dental caries .
- Development of dental materials: Amelogenin is used in the creation of biomimetic materials for dental restorations and repairs .
