Polcalcin Aln g 4 is a glycosylated polypeptide chain with a calculated molecular mass of approximately 10,185 Daltons . The recombinant version is typically produced in insect cells (SF9) and is expressed with a 6xHis tag at the N-terminus to facilitate purification . The protein is purified using proprietary chromatographic techniques to ensure high purity and functionality.
Polcalcin Aln g 4 belongs to the family of calcium-binding proteins known as polcalcins. These proteins play a crucial role in the regulation of intracellular calcium levels, which is essential for various cellular processes. In the context of allergies, polcalcins like Aln g 4 are recognized by the immune system of sensitive individuals, leading to an allergic response. This response can manifest as symptoms such as sneezing, runny nose, and itchy eyes.
The recombinant form of Polcalcin Aln g 4 is used extensively in research to understand the molecular mechanisms underlying pollen allergies. By studying the structure and function of this protein, scientists aim to develop better diagnostic tools and therapeutic strategies for managing pollen-induced allergic reactions. Additionally, recombinant Polcalcin Aln g 4 is used in immunotherapy research to desensitize allergic individuals to pollen allergens.