Aln G 4.0101

Polcalcin Aln g 4 Recombinant
Cat. No.
BT15646
Source
Sf9 insect cells.
Synonyms
Polcalcin Aln g 4, Calcium-binding pollen allergen Aln g 4, Aln g 4.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Recombinant Polcalcin Aln g 4 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 10,185 Dalton.
Aln G 4.0101 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Polcalcin Aln g 4, also known as Aln G 4.0101, is an allergen that can trigger allergic responses in humans.
Description
Recombinant Polcalcin Aln g 4, a glycosylated polypeptide chain with a calculated molecular mass of 10,185 Daltons, is produced in SF9 cells. This protein (Aln G 4.0101) is engineered with a 6xHis tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
Aln G 4.0101 is provided in a solution of 20mM HEPES buffer at pH 7.9 and 6M Urea.
Stability
For short-term storage (up to 2-4 weeks), store the vial at 4°C. For extended storage, freeze at -20°C. It is advisable to avoid repeated freeze-thaw cycles.
Purity
SDS-PAGE analysis indicates a purity greater than 80.0%.
Immunological Functions
1. Demonstrates the ability to bind IgE antibodies in humans. 2. Suitable for use in immunodot assays employing positive and negative serum panels.
Synonyms
Polcalcin Aln g 4, Calcium-binding pollen allergen Aln g 4, Aln g 4.
Source
Sf9 insect cells.

Product Science Overview

Structure and Production

Polcalcin Aln g 4 is a glycosylated polypeptide chain with a calculated molecular mass of approximately 10,185 Daltons . The recombinant version is typically produced in insect cells (SF9) and is expressed with a 6xHis tag at the N-terminus to facilitate purification . The protein is purified using proprietary chromatographic techniques to ensure high purity and functionality.

Function and Role in Allergies

Polcalcin Aln g 4 belongs to the family of calcium-binding proteins known as polcalcins. These proteins play a crucial role in the regulation of intracellular calcium levels, which is essential for various cellular processes. In the context of allergies, polcalcins like Aln g 4 are recognized by the immune system of sensitive individuals, leading to an allergic response. This response can manifest as symptoms such as sneezing, runny nose, and itchy eyes.

Applications in Research

The recombinant form of Polcalcin Aln g 4 is used extensively in research to understand the molecular mechanisms underlying pollen allergies. By studying the structure and function of this protein, scientists aim to develop better diagnostic tools and therapeutic strategies for managing pollen-induced allergic reactions. Additionally, recombinant Polcalcin Aln g 4 is used in immunotherapy research to desensitize allergic individuals to pollen allergens.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2024 Thebiotek. All Rights Reserved.